WIN18446, 1477-57-2, IC-01228393
Product Introduction
WIN18446 is a potent and irreversible inhibitor of the aldehyde dehydrogenase 1A (ALDH1A) family of enzymes, with a reported IC50 of 0.3 µM against ALDH1A2. ALDH1A2 catalyzes the final step in retinoic acid biosynthesis from retinol (vitamin A), and its inhibition by WIN18446 effectively blocks all-trans retinoic acid (atRA) production in target tissues. WIN18446 has been widely used as a pharmacological tool to study retinoid signaling, spermatogenesis, and male fertility regulation. In vivo, WIN18446 reversibly suppresses spermatogenesis in multiple species, including rabbits and mice, by depleting intratesticular retinoic acid, and fertility recovers after treatment cessation. The compound has also been investigated for its effects on spermatogonial stem cell transplantation and blood-testis barrier permeability.
Product Features
1. Potent ALDH1A Inhibitor: Irreversibly inhibits ALDH1A family enzymes, including ALDH1A2 (IC50 = 0.3 µM).
2. Blocks Retinoic Acid Biosynthesis: Prevents the conversion of retinol to all-trans retinoic acid, enabling study of retinoid-dependent processes.
3. Reversible Male Contraceptive Tool: Suppresses spermatogenesis in vivo, with fertility recovery upon treatment cessation.
4. Widely Validated Research Tool: Used in studies of spermatogenesis synchronization, germline stem cell transplantation, and retinoid metabolism.
5. High Purity: Supplied at 98% purity for reproducible pharmacological studies.
Specifications
Size: 50 mg / 100 mg / 1 mL (10 mM in DMSO)
CAS Number: 1477-57-2
Purity: 98%
Molecular Weight: 366.11
Molecular Formula: C12H20Cl4N2O2
Solubility: DMSO: 100 mg/mL (273.14 mM; sonication-assisted dissolution <60¡ãC); limited solubility in aqueous buffers
Storage and Stability
Storage Conditions: Store at 2-8¡ãC under inert atmosphere, protected from light and moisture.
Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed. For reconstituted solutions in DMSO, aliquot and store at -20¡ãC protected from light. Avoid repeated freeze-thaw cycles.
Protocol (For Reference Only)
Important: WIN18446 is moisture-sensitive. Use freshly opened anhydrous DMSO for stock solution preparation. Protect from light during handling and storage.
1. Stock Solution Preparation: Dissolve WIN18446 in anhydrous DMSO to prepare a concentrated stock solution (e.g., 10-50 mM). Sonication (<60¡ãC) may be used to assist dissolution. Aliquot into single-use vials and store at -20¡ãC protected from light.
2. In Vitro Working Solution Preparation: Dilute DMSO stock solution into culture medium or assay buffer to achieve working concentrations typically ranging from 0.1-10 µM. Limit final DMSO concentration to ¡Ü0.1%.
3. ALDH1A2 Inhibition Assay: For in vitro enzyme inhibition studies, incubate ALDH1A2 with WIN18446 at concentrations around the IC50 (0.3 µM) and measure residual enzyme activity using retinoic acid formation as a readout.
4. Spermatogenesis Synchronization (In Vivo): For studies requiring synchronized spermatogenesis, WIN18446 has been administered to rodents to halt retinoic acid synthesis and prevent meiotic commitment of spermatogonia, followed by exogenous atRA treatment to resume synchronized spermatogenesis.
5. Retinoid Metabolism Studies: Use WIN18446 to block retinoic acid biosynthesis in cell or tissue models and assess effects on retinoid-dependent gene expression and metabolism.
Precautions
1. Protect from light and moisture during handling and storage.
2. Use freshly opened anhydrous DMSO for stock solutions; moist DMSO significantly reduces solubility.
3. Use appropriate vehicle controls in all experiments; DMSO concentration in culture medium should not exceed 0.1%.
4. WIN18446 inhibits ALDH2 at higher concentrations or with prolonged exposure; interpret results with consideration of potential effects on acetaldehyde metabolism.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the primary target of WIN18446?
A1: WIN18446 is a potent irreversible inhibitor of the ALDH1A family, with an IC50 of 0.3 µM against ALDH1A2.
Q2: How does WIN18446 affect spermatogenesis?
A2: WIN18446 blocks retinoic acid biosynthesis in the testis, preventing the commitment of undifferentiated spermatogonia to meiosis and reversibly suppressing spermatogenesis.
Q3: How should I prepare a stock solution of WIN18446?
A3: Dissolve in freshly opened anhydrous DMSO to prepare a concentrated stock solution. Sonication (<60¡ãC) may be used to assist dissolution. Moist DMSO significantly reduces solubility.
Q4: Is the suppression of spermatogenesis by WIN18446 reversible?
A4: Yes. In animal studies, sperm counts and fertility recovered after WIN18446 treatment was discontinued.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of conditions using a small-scale pilot experiment is recommended before processing valuable samples.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-01228393
Product Name: WIN18446
Size: 50 mg / 100 mg / 1 mL (10 mM in DMSO)
Suggested Retail Price:
50 mg: CNY ¥850.00 / USD $85.00 / EUR €102.00 / JPY ¥15300.00
1 mL (10 mM in DMSO): CNY ¥1485.00 / USD $148.50 / EUR €178.20 / JPY ¥26730.00
100 mg: CNY ¥1820.00 / USD $182.00 / EUR €218.40 / JPY ¥32760.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
