Dextran Sulfate Sodium (MW 40,000), IC-0360940
Product Introduction
Dextran sulfate sodium (DSS) with a molecular weight of approximately 40,000 Da is a sulfated polysaccharide derived from dextran, composed of glucose units with sulfate groups attached. DSS is widely used in biomedical research as an inducer of experimental colitis in rodents, a well-established model for studying inflammatory bowel disease (IBD). Administration of DSS in drinking water at concentrations of 1-5% (w/v) for 5-10 days induces acute colitis characterized by epithelial damage, crypt loss, mucosal inflammation, and ulceration, with clinical features resembling human ulcerative colitis. The severity of colitis depends on DSS concentration, molecular weight, administration duration, and mouse strain. DSS is also used as an anticoagulant, a polyanion for nucleic acid hybridization, and a component in virus adsorption and plaque assays. The 40,000 Da molecular weight form is commonly used for colitis induction and other biomedical applications.
Product Features
1. Colitis Induction: Widely used to induce experimental colitis in rodents as a model of inflammatory bowel disease.
2. Reproducible Model: Well-characterized and reproducible colitis induction with dose- and time-dependent severity.
3. Versatile Applications: Also used as an anticoagulant, hybridization reagent, and in virus plaque assays.
4. High Purity: Supplied at research-grade purity for reproducible experimental results.
5. Defined Molecular Weight: MW ~40,000 Da suitable for colitis models and other applications.
Specifications
Size: 1 g / 5 g / 10 g / 50 g
CAS Number: 9011-18-1
Molecular Weight: ~40,000 Da
Molecular Formula: (C6H7Na3O14S3)n
Solubility: Water: readily soluble
Appearance: White to off-white powder
Storage and Stability
Storage Conditions: Store at room temperature under inert atmosphere, protected from moisture.
Shelf Life: The product is stable for 36 months from the date of manufacture when stored as directed.
Protocol (For Reference Only)
Important: DSS is hygroscopic. Store under inert atmosphere and keep container tightly sealed.
1. Colitis Induction in Mice: Prepare DSS solution fresh in autoclaved drinking water at 1-5% (w/v). Filter sterilize if required. Administer ad libitum to mice for 5-10 days depending on the desired severity of colitis.
2. Acute Colitis Protocol: Administer 2-3% (w/v) DSS in drinking water for 7 days, followed by 2-3 days of regular water. Monitor body weight, stool consistency, and fecal bleeding daily.
3. Chronic Colitis Protocol: Administer 2-3% (w/v) DSS in drinking water for 5-7 days followed by 14 days of regular water. Repeat cycles 2-3 times to induce chronic colitis.
4. Assessment: Evaluate colitis severity by disease activity index (DAI), colon length, histological scoring, and inflammatory cytokine measurement.
5. Other Applications: For anticoagulant use, prepare DSS solutions at appropriate concentrations in physiological buffer. For hybridization applications, use at 0.1-1% (w/v) in hybridization buffer.
Precautions
1. DSS solution should be prepared fresh before use and replaced every 2-3 days during the induction period.
2. The severity of colitis depends on DSS concentration, molecular weight, duration of administration, and mouse strain. Optimize conditions for your specific model.
3. Monitor animal body weight and clinical signs daily during DSS administration.
4. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the typical DSS concentration for colitis induction?
A1: Typical concentrations range from 1-5% (w/v) in drinking water. For acute colitis, 2-3% for 7 days is commonly used. Optimize based on mouse strain and desired severity.
Q2: How should I prepare DSS solution?
A2: Prepare fresh DSS solution in autoclaved drinking water. Filter sterilize if required. Replace solution every 2-3 days during the induction period.
Q3: How long does it take to induce colitis?
A3: Acute colitis typically develops within 5-10 days of DSS administration. Clinical signs include weight loss, diarrhea, and bloody stool.
Q4: What molecular weight of DSS is recommended for colitis induction?
A4: DSS with molecular weights of 36,000-50,000 Da is commonly used for colitis induction. This product has a molecular weight of approximately 40,000 Da.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of animal experiments, optimization of DSS concentration and administration duration is recommended for specific mouse strains and experimental models.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-0360940
Product Name: Dextran Sulfate Sodium (MW 40,000)
Size: 1 g / 5 g / 10 g / 50 g
Suggested Retail Price:
1 g: CNY ¥260.00 / USD $26.00 / EUR €31.20 / JPY ¥4680.00
5 g: CNY ¥390.00 / USD $39.00 / EUR €46.80 / JPY ¥7020.00
10 g: CNY ¥650.00 / USD $65.00 / EUR €78.00 / JPY ¥11700.00
50 g: CNY ¥2550.00 / USD $255.00 / EUR €306.00 / JPY ¥45900.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
