Rhodamine B Isothiocyanate–Dextran, IC-0195358
Product Description
Rhodamine B isothiocyanate–dextran is a fluorescently labeled polymer formed by the covalent conjugation of dextran (average molecular weight ~10,000) with rhodamine B isothiocyanate (RITC).
Rhodamine B is a sulfhydryl-reactive fluorescent dye with excellent photostability. Its derivative, rhodamine B isothiocyanate, belongs to the amino-xanthene class of dyes. Its molecular structure features a benzene ring substituted with both a carboxylic acid group and a reactive isothiocyanate group (-N=C=S). The isothiocyanate group can react with amino groups on biomolecules (e.g., proteins, polysaccharides) to form stable thiourea bonds, enabling efficient and reliable fluorescent labeling.
This product is a water-soluble fluorescent tracer with a labeling ratio of 0.002–0.01 mol RITC per mol glucose unit. It forms a clear pink solution in water and emits bright red fluorescence.
Product Information
- Chemical Composition: Rhodamine B isothiocyanate-labeled dextran conjugate.
- Average Molecular Weight: ~10,000
- Labeling Ratio: 0.002–0.01 mol RITC per mol glucose unit
- Ex/Em Wavelength: Approximately ~560 nm / ~580 nm (specific values may vary depending on the environment and instrument).
- Appearance: Powder
- Color: Light pink to red or very dark purple solid powder
- Solubility: Soluble in water at 10 mg/mL, forming a clear, strongly fluorescent pink solution.
- Storage Conditions: Store sealed and protected from light in a refrigerator at 2–8°C to maintain long-term stability.
Application Information
This product is a versatile fluorescent tracer widely used in biomedical research, including:
- Cell Biology: Commonly used as a fluorescent probe to study intracellular activities such as endocytosis, lysosomal transport, and intracellular pH changes. It is also used to detect and track the migration of labeled cells (e.g., in cancer metastasis or immune cell chemotaxis studies).
- Neuroscience: Suitable for perfusion studies in animals, such as evaluating vascular permeability, microcirculation, and blood perfusion. It can be used for in vivo dye diffusion assays to assess the integrity and function of the blood-brain barrier (BBB) in zebrafish.
- Histology and Hematology: Used for labeling and tracing tissues, blood vessels, and cells in fluorescence microscopy techniques. Rhodamine B stains tissues a purplish-red color and emits bright red fluorescence, making it also suitable for ecological studies such as insect labeling.
Biochemical/Physiological Effects
The rhodamine B dye component possesses unique optical properties, including strong fluorescence and high photostability. Under physiological conditions, as a macromolecular tracer, its cellular permeability and in vivo distribution are primarily controlled by the molecular weight of the dextran backbone. Dextran with a molecular weight of 10,000 can enter cells via endocytic pathways and has a moderate retention time in the vascular system, making it highly suitable for studying vascular leakage, phagocytosis, and barrier functions (e.g., blood-brain barrier).
Storage
Store in the original container, tightly sealed. Keep in a dry, dark, and cool (2–8°C) environment. Prolonged exposure to light may cause fluorescence quenching.
Safety Information
- Safety Note: This product is a chemical and should be handled as a potential health hazard.
- Usage Instructions: This product is intended for research use only. It is strictly prohibited for medicinal, clinical, or household use.
Order Information
|
Cat./REF. |
Size |
Price($） |
Price(�) |
Price(￥/CNY） |
Price(￥/JYP） |
|
IC-0195358 |
100mg |
$550.00 |
� 660.00 |
￥5,500.00 |
￥109,450.00 |
|
IC-0195358 |
500mg |
$2,200.00 |
� 2,640.00 |
￥22,000.00 |
￥437,800.00 |
