25 cm² Cell Culture Flask with Vent Cap, IC-30025
Product Introduction
This product is a 25 cm² cell culture flask with a vent cap, manufactured under Class C cleanroom conditions using automated equipment in accordance with cGMP standards. The production process ensures sterility, pyrogen-free status, and the absence of animal-derived components. The flask surface is treated using an ultra-vacuum plasma surface modification process (TC treatment) to enhance cell attachment and support the growth of low-adherence cell types.
Product Features
1. cGMP Manufacturing: Produced under Class C cleanroom conditions using automated equipment in accordance with cGMP standards.
2. TC Surface Treatment: Ultra-vacuum plasma surface modification enhances cell attachment and supports low-adherence cell growth.
3. Curved Bottom Design: The rounded bottom corners reduce dead zones during pipetting.
4. Dual Cap Options: Standard configuration includes both 0.2 µm hydrophobic vent caps and sealed caps for different culture environments.
5. Hydrophobic Vent Membrane: The vent membrane is hydrophobized so that liquid contact does not affect sealing or gas exchange.
6. Bottom Vent Holes: Vent holes in the bottom support allow rapid gas exchange within the incubator, shortening the rewarming time after transfer from room temperature.
Specifications
Surface Area: 25 cm²
Cap Type: Vent cap (0.2 µm hydrophobic membrane) and sealed cap
Packaging: 10 flasks per pack, 200 flasks per case
Material: Tissue culture treated polystyrene
Sterility: Sterile, pyrogen-free, no animal-derived components
Storage and Stability
Storage Conditions: Store at room temperature in a dry, clean environment. Protect from dust and direct sunlight.
Shelf Life: The product is stable for 36 months from the date of manufacture when stored as directed in unopened packaging.
Protocol (For Reference Only)
Important: Use aseptic technique when opening packaging and handling flasks. Select the appropriate cap type based on the culture requirements.
1. Preparation: Open the packaging in a biosafety cabinet. Label the flask appropriately.
2. Cell Seeding: Add the desired volume of cell suspension in complete culture medium (typically 5-7 mL for a 25 cm² flask). Gently rock the flask to distribute cells evenly.
3. Incubation: Place the flask in a 37¡ãC, 5% CO₂ incubator. Use the vent cap for standard culture to allow gas exchange. Use the sealed cap when a closed system is required.
4. Medium Change: Aspirate spent medium, wash with PBS if desired, and add fresh pre-warmed complete medium.
5. Subculturing: When cells reach the desired confluency, detach using trypsin-EDTA and passage according to standard protocols.
Precautions
1. Use aseptic technique to maintain sterility after opening.
2. Handle flasks by the edges to avoid touching the cell growth surface.
3. Select the appropriate cap type based on the culture requirements.
4. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the difference between the vent cap and the sealed cap?
A1: The vent cap contains a 0.2 µm hydrophobic membrane that allows gas exchange while preventing microbial contamination, suitable for standard culture in CO₂ incubators. The sealed cap provides a closed environment, suitable for anaerobic culture or transport.
Q2: What surface treatment is applied to this flask?
A2: The flask is treated with ultra-vacuum plasma surface modification (TC treatment) to enhance cell attachment.
Q3: What is the recommended medium volume for a 25 cm² flask?
A3: The typical working volume is 5-7 mL of culture medium.
Q4: Is this product free of animal-derived components?
A4: Yes. The product is manufactured without animal-derived components.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
3. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-30025
Product Name: 25 cm² Cell Culture Flask with Vent Cap
Size: 10 flasks per pack, 200 flasks per case
Suggested Retail Price:
Per Pack (10 flasks): CNY ¥55.00 / USD $5.50 / EUR €6.60 / JPY ¥990.00
Per Case (200 flasks): CNY ¥1100.00 / USD $110.00 / EUR €132.00 / JPY ¥19800.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
