100 mm Cell Culture Dish, IC-30100
Product Introduction
This product is a 100 mm cell culture dish manufactured from high-quality transparent polystyrene (PS) conforming to USP Class VI medical-grade standards. Cell culture dishes are essential consumables for routine adherent cell culture, providing a flat growth surface with optimal optical properties for microscopic observation. The dish features uniform thickness, distortion-free bottom, and an easy-grip flat base design with a serrated ring on the side to minimize contamination risk. The dish can be used with an open-style lid featuring regular protrusions for gas exchange. The surface has been treated by vacuum plasma to provide excellent cell attachment properties. This product is certified free of pyrogens, endotoxins, and cytotoxicity. Sterilized by irradiation to a sterility assurance level (SAL) of 10⁻⁶. The annular protrusion on the lid fits tightly with the base, facilitating storage and reducing medium evaporation.
Product Features
1. Medical-Grade Material: High-quality transparent polystyrene conforming to USP Class VI standards.
2. Uniform Thickness: Even bottom thickness with no distortion, ensuring consistent optical properties for microscopy.
3. Vacuum Plasma Treated: Surface treatment provides excellent cell attachment and growth.
4. Easy-Grip Design: Serrated ring on the side for secure handling and reduced contamination risk.
5. Sterile and Pyrogen-Free: Irradiation sterilized, SAL 10⁻⁶. Free of pyrogens, endotoxins, and cytotoxicity.
Specifications
Size: 100 mm
Packaging: 10 dishes per pack, 300 dishes per case
Material: Transparent polystyrene (PS), USP Class VI
Surface Treatment: Vacuum plasma treated for cell attachment
Sterilization: Irradiation, SAL 10⁻⁶
Quality: Pyrogen-free, endotoxin-free, non-cytotoxic
Storage and Stability
Storage Conditions: Store at room temperature in a dry, clean environment. Protect from dust and direct sunlight.
Shelf Life: The product is stable for 36 months from the date of manufacture when stored as directed in unopened packaging.
Protocol (For Reference Only)
Important: Use aseptic technique when opening the packaging and handling dishes to maintain sterility.
1. Preparation: Open the packaging in a biosafety cabinet. Remove the dish and label appropriately.
2. Cell Seeding: Add the desired volume of cell suspension in complete culture medium (typically 8-12 mL for a 100 mm dish). Gently swirl to evenly distribute cells.
3. Incubation: Place the dish in a 37¡ãC, 5% CO₂ incubator. For optimal gas exchange, use the lid with protrusions in the open position.
4. Medium Change: Aspirate spent medium, wash with PBS if desired, and add fresh pre-warmed complete medium.
5. Subculturing: When cells reach desired confluency, detach using trypsin-EDTA and passage according to standard protocols.
Precautions
1. Use aseptic technique to maintain sterility after opening.
2. Handle dishes by the edges to avoid touching the cell growth surface.
3. Stack dishes carefully in the incubator to ensure proper gas exchange.
4. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What surface treatment is applied to this dish?
A1: The dish surface is treated by vacuum plasma to ensure excellent cell attachment for adherent cell culture.
Q2: What is the recommended medium volume for a 100 mm dish?
A2: The typical working volume is 8-12 mL of culture medium.
Q3: Is this dish sterile?
A3: Yes. The dish is sterilized by irradiation to SAL 10⁻⁶ and is certified pyrogen-free, endotoxin-free, and non-cytotoxic.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
3. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-30100
Product Name: 100 mm Cell Culture Dish
Size: 10 dishes per pack, 300 dishes per case
Suggested Retail Price:
Per Pack (10 dishes): CNY ¥30.00 / USD $3.00 / EUR €3.60 / JPY ¥540.00
Per Case (300 dishes): CNY ¥900.00 / USD $90.00 / EUR €108.00 / JPY ¥16200.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
