Recombinant Mouse IL-2, CYT-P04351
Product Introduction
Interleukin-2 (IL-2) is a 15-17 kDa pleiotropic cytokine primarily produced by activated CD4+ T cells, CD8+ T cells, and dendritic cells. IL-2 plays a central role in the adaptive immune response by promoting the proliferation, differentiation, and survival of T cells, B cells, and natural killer (NK) cells. IL-2 signals through the IL-2 receptor complex consisting of the IL-2R¦Á (CD25), IL-2R¦Â (CD122), and common gamma chain (¦Ãc/CD132) subunits. At low concentrations, IL-2 preferentially expands regulatory T cells (Tregs) due to their constitutive high expression of the high-affinity trimeric IL-2 receptor, whereas at higher concentrations, IL-2 drives the expansion and effector function of CD8+ T cells and NK cells. Recombinant Mouse IL-2 is extensively utilized in immunology research for T cell expansion and culture, in vitro Treg generation and maintenance, NK cell activation, and preclinical studies investigating IL-2-based immunotherapies and autoimmune disease mechanisms.
Product Features
1. High Biological Activity: Potently stimulates proliferation of mouse CTLL-2 cells with an ED₅₀ typically in the low ng/mL range.
2. Recombinant Mouse Protein: Produced in E. coli as a non-glycosylated single polypeptide chain representing the mature form of mouse IL-2.
3. High Purity: Supplied at ¡Ý95% purity as determined by SDS-PAGE and HPLC analysis.
4. Endotoxin-Tested: Low endotoxin level suitable for sensitive primary cell culture and in vivo applications.
5. Carrier-Free Option Available: Lyophilized from a sterile-filtered solution without carrier protein for applications requiring minimal interference.
Specifications
Size: Available in 10 µg and 50 µg
Source: E. coli
Molecular Weight: 17.2 kDa (mature form, non-glycosylated)
Purity: ¡Ý95% by SDS-PAGE
Endotoxin: <0.1 EU/µg (LAL assay)
Biological Activity: ED₅₀ ¡Ü5 ng/mL as measured by CTLL-2 cell proliferation assay
Formulation: Lyophilized from a sterile-filtered solution in PBS, pH 7.4
Solubility: Reconstitute in sterile water or aqueous buffer to a concentration of 0.1-1.0 mg/mL
Storage and Stability
Storage Conditions: Lyophilized protein should be stored at -20¡æor -80¡ãC. Upon reconstitution, aliquot and store at -20¡æor -80¡ãC. Avoid repeated freeze-thaw cycles.
Shelf Life: The lyophilized product is stable for 12 months from the date of manufacture when stored as directed. Reconstituted product is stable for up to 3 months when stored at -80¡ãC.
Protocol (For Reference Only)
Important: Centrifuge the vial briefly before opening to collect lyophilized contents. Reconstitute under aseptic conditions. Avoid vigorous vortexing.
1. Reconstitution: Briefly centrifuge the vial to collect the lyophilized powder. Reconstitute Recombinant Mouse IL-2 in sterile deionized water or sterile PBS to a concentration of 0.1-1.0 mg/mL. Allow the solution to sit at room temperature for 10-15 minutes with gentle swirling to ensure complete dissolution.
2. Dilution for Working Solutions: Following reconstitution, dilute the stock solution in cell culture medium or buffer containing carrier protein (e.g., 0.1% BSA) to prevent non-specific adsorption to plastic surfaces. Do not dilute below 1 µg/mL without carrier protein.
3. T Cell Proliferation and Expansion: Supplement mouse T cell culture medium with Recombinant Mouse IL-2 at a final concentration of 1-20 ng/mL for CTLL-2 cell proliferation or 5-50 ng/mL for primary mouse T cell expansion and maintenance.
4. NK Cell Activation: For NK cell stimulation, add Recombinant Mouse IL-2 to culture medium at a final concentration of 10-100 ng/mL, alone or in combination with IL-12 or IL-15, and incubate for 24-72 hours before assessing cytotoxicity or cytokine production.
5. Treg Expansion: For mouse Treg generation or expansion, supplement cultures with Recombinant Mouse IL-2 at a final concentration of 2-10 ng/mL in combination with TGF-¦Â and anti-CD3/CD28 stimulation for in vitro Treg differentiation, or at 100-500 IU/mL for ex vivo Treg expansion.
Precautions
1. Reconstituted protein is less stable than the lyophilized form; aliquot and store at -80¡æimmediately after reconstitution.
2. Avoid repeated freeze-thaw cycles as this will significantly reduce biological activity.
3. For dilutions below 1 µg/mL, always add a carrier protein (0.1% BSA or 10% FBS) to prevent non-specific adsorption of IL-2 to container surfaces.
4. Optimal working concentrations should be determined empirically for each cell type and experimental system.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the recommended IL-2 concentration for CTLL-2 cell culture?
A1: Recombinant Mouse IL-2 at 1-20 ng/mL effectively supports CTLL-2 cell proliferation. For routine maintenance of CTLL-2 cells, 2-5 ng/mL is typically sufficient. Titration in your specific culture system is recommended.
Q2: How should I store this product after reconstitution?
A2: Aliquot reconstituted IL-2 into single-use volumes and store at -80¡ãC. Avoid storage at 4¡æfor more than one week. Repeated freeze-thaw cycles will significantly reduce activity.
Q3: Can I use this product in in vivo experiments?
A3: Yes. This recombinant protein is produced under conditions yielding low endotoxin levels suitable for in vivo administration. Typical in vivo doses in mouse studies range from 1-50 µg/kg depending on the experimental model and route of administration.
Q4: What is the difference between mouse and human IL-2?
A4: Mouse IL-2 shares approximately 60% amino acid sequence identity with human IL-2. Mouse IL-2 is active on mouse cells but exhibits significantly reduced cross-species activity on human cells, and vice versa. For mouse immunology studies, recombinant mouse IL-2 is recommended.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of cytokine concentration and culture conditions is strongly recommended for specific cell types and experimental endpoints.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: CYT-P04351
Product Name: Recombinant Mouse IL-2
Size: 10 µg / 50 µg
Price:
10 µg: CNY ¥1450.00 / USD $145.00 / EUR €180.00 / JPY ¥27000.00
50 µg: CNY ¥3500.00 / USD $350.00 / EUR €420.00 / JPY ¥63000.00
2023 Version
