Mesenchymal Stem Cell Medium,MSCM£¨CC-10010£©

(Apply For MSC Culture)

BACKGOUND 

Mesenchymal Stem Cell Medium (MSCM), when used with Mesenchymal Stem Cell Growth Supplement (MSCGS, CYT-1011) and 25 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal mesenchymal stem cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal mesenchymal stem cells in vitro.

Content
MSCM consists of 500 ml of basal medium, 25 ml of FBS (IC-1908), 5 ml of Mesenchymal Stem Cell Growth Supplement (MSCGS,CYT-1011) and 5 ml of penicillin/streptomycin solution (P/S, 0110-AP). Note: FBS, MSCGS and P/S are not pre-mixed in MSCM; they must be added separately to make the complete MSCM.

Size
500ML

Shipping

Basal medium: room temperature. Supplements: dry ice.

Usage
Thaw MSCGS, FBS and P/S solution at 37¡æ. Gently tilt the tubes several times to ensure the contents are completely mixed before adding to the medium. Spray the medium bottle and tubes with 70% ethanol, and wipe to remove excess liquid. In a sterile field, remove the caps without touching the interior threads with fingers. Add MSCGS, FBS and P/S solution to the medium and mix well. Since several components are light-labile, the medium should not be exposed to light for extended periods. We do not recommend warming medium in a 37¡æ water bath prior to use. When stored in the dark at 4¡æ, the reconstituted medium is stable for one month.

STORAGE

Store the basal medium at 2-8¡æ and the MSCGS, FBS and P/S solution at -20¡æ. Protect from light.

Note: For your safety and health, please wear experimental clothes and disposable gloves.This product is for scientific research only.MSCM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.