Medium 199 (M199)，IC-2039-S
Product Background
Medium 199 (M199) is a complex synthetic medium originally developed for the nutritional study of chicken embryo cells. It is now widely used for culturing a broad range of mammalian cell types, including primary cells, endothelial cells, and virus production. M199 contains a balanced mixture of amino acids, vitamins, nucleotides, and other growth factors, making it suitable for specialized applications requiring enriched conditions.
Key Features
- Comprehensive Formulation:
- Amino Acids: Includes all 20 essential and non-essential amino acids.
- Vitamins: 15+ vitamins (e.g., A, D, E, K, B-complex).
- Additional Components: Nucleotides (adenine, guanine, uracil), cholesterol, and Tween 80.
- Flexible Base:
- Available with or without phenol red; HEPES or bicarbonate buffering options.
- Glucose-free and low-serum formulations upon request.
- Physical Properties:
- Osmolality: 280–320 mOsm/kg.
- pH: 7.0–7.4 (with 5% CO₂ equilibration).
Usage Instructions
1. Preparation:
- Powdered M199: Dissolve in distilled water, adjust pH to 7.2–7.4, and sterilize by filtration (0.22 µm).
- Liquid M199: Thaw at 4°C and pre-warm to 37°C before use.
2. Supplementation:
- Serum: Add 5–10% FBS or species-specific serum for primary cells.
- Growth Factors: EGF, bFGF, or heparin may be required for endothelial cultures.
- Antibiotics: Optional 1% penicillin/streptomycin.
3. Cell Culture:
- Use for adherent or suspension cultures at 37°C with 5% CO₂.
- For virus production (e.g., vaccines), supplement as per protocol.
Quality Control
- Sterility: Tested per USP/EP standards (no microbial growth after 14 days).
- Performance: Supports ≥90% viability of human umbilical vein endothelial cells (HUVECs) over 96 hours.
- Endotoxin: <0.25 EU/mL (LAL test).
- Consistency: Each lot tested for osmolality, pH, and nutrient stability.
Precautions
1. Storage:
- Liquid: 2–8°C (avoid repeated freeze-thaw cycles).
- Powder: Room temperature (15–25°C) in a dry environment.
2. Handling:
- Avoid prolonged exposure to light (vitamin-sensitive).
- Discard if color changes (pink to yellow indicates pH shift).
3. Safety:For research use only. Not intended for therapeutic applications.
4. Compatibility: Verify compatibility with additives (e.g., reducing agents may degrade vitamins).
Order Information
|
Cat./REF. |
Size |
Price($） |
Price(�) |
Price(￥/CNY） |
Price(￥/JYP） |
|
IC-2039-S |
500ml |
$26.00 |
� 31.20 |
￥260.00 |
￥5,174.00 |
