Contaminant Scavenger,CP-CS-0410

Contaminant Scavenger

CP-CS-0410

Product Description

The high-efficiency cell pollutant remover aims to comprehensively protect cells from microbial contamination, not only significantly removing mycoplasma contamination, but also

It can effectively eliminate contamination from fungi and bacteria, and can be used throughout the entire cultivation process. It can be added to the culture medium or used for tissue cleaning. It only takes 1-3 days to significantly inhibit the proliferation of fungi, bacteria, and mycoplasma, and about 1-2 weeks to remove contamination. This product has been tested on hundreds of cells and verified through long-term experiments, perfectly combining the ability to efficiently and broad-spectrum remove cellular pollutants, with almost no impact on cell growth, and maximizing the rescue of your precious cells.

Usage method

1.Take out the high-efficiency cell pollutant remover from the -20 ¡æ refrigerator, centrifuge the reagent tube instantaneously (3000rpm, 3-5 seconds), place it on the EP tube rack, spray the surface of the reagent tube with 75% alcohol, and perform aseptic operation in the biosafety cabinet.

2.Operating Procedures for Removing Cell Contamination: Taking T25 Cell Culture Bottle as an Example:

2.1 Due to the difficulty in detecting cell contamination in the early stages, if the cells are fragile, such as embryonic stem cells (H1, H9, iPS), it is recommended to use a dilution factor of 2000. For example, 6mL of complete culture medium should be mixed with 3 ¦Ì L of highly efficient cell pollutant remover, and continuously cultured for 1-2 weeks (or passaged 3 times) to detect the presence of contamination.

2.2 For conventional cells (cell lines, primary cells, stem cells), if there are obvious signs of cell contamination, it is necessary to increase the drug concentration as appropriate to improve the efficiency of pollutant removal. It is recommended to increase the concentration to 1000 x to remove cell pollutants, such as adding 6 ¦Ì L of high-efficiency cell pollutant remover to 6mL of complete culture medium and mixing well. Change the liquid twice a day. It is recommended to enter the laboratory in the morning to change the medium with one dose. In the afternoon, change the medium with one dose before leaving the laboratory. Repeat the process on the second day and the third day. Change the liquid once a day from the the fourth day. After continuous dosing and culture for 1-2 weeks (or passage for 3 times), check whether there is still pollution.

2.3 Note:

¢Ù Before changing the medium or passaging the adherent cells, discard the old culture medium, rinse off as much contaminants as possible with PBS, and then wash the cell surface twice with PBS.

¢Ú If the cells reach the passable ratio, please passage them in a timely manner and place them in a new cell culture bottle. The process of replacing the bottle can also effectively avoid contamination by residual microorganisms on the wall of the culture bottle;

¢Û After the pollution is cleared, it must be passaged and placed in a new cell culture bottle. After adding medication to the new bottle for 1-2 days, the high-efficiency cleaning agent for cell pollution can be removed to avoid secondary pollution caused by residual pollutants on the wall of the old bottle after removing the medication from the old bottle;

3.Prevention of Cell Contamination Operating Procedures: Taking T25 Cell Culture Bottle as an Example

3.1 If cells need to be cultured continuously, it is recommended to take regular preventive measures every 2-3 weeks. Add an appropriate amount of high-efficiency cell pollutant remover to the cell culture medium, and the recommended dilution ratio is 3000 times. For example, mix 6mL of cell culture medium with 2 ¦Ì L of high-efficiency cell pollutant remover. Continuous drug addition and cultivation for one week can effectively prevent contamination of cells by fungi, bacteria, mycoplasma, and black glue worms.

3.2 The operating procedures for cleaning tissues are essential for primary cell culture of easily contaminated tissues in the digestive, reproductive, urinary, and skin systems. Aseptic tissue is a prerequisite for cell culture.

3.3 After separating the target tissue, clean the blood, hair, and dirt thoroughly with PBS. Prepare an appropriate amount of PBS containing 1000 x cell contamination efficient scavenger, such as mixing 10mL of PBS with 10 ¦Ì L of cell contamination efficient scavenger. Soaking tissues in medicated PBS for 2-5 minutes and washing them three times can effectively prevent contamination by fungi, bacteria, mycoplasma, and black glue worms.

Transportation and storage methods

Ice pack transportation. Store at 20 ¡æ away from light, with a shelf life of 2 years.

Product advantages

1.High efficiency, can effectively inhibit the proliferation of fungi, bacteria, mycoplasma, and black glue worms within one day;

2.High specificity, specifically clearing pollutants during cell culture process;

3.No drug resistance, active ingredients are peptides, and no drug resistance will occur;

4.High utilization rate, unused components can be degraded into amino acids and utilized by cells;

5.High safety, almost non-toxic to cells, has been validated on hundreds of cell types.

Important

1. Please read the instructions carefully before using this reagent;

2. This product is sterilized by 0.1 ¦Ì m filtration, and does not require filtration when using it. It can be directly added to the culture medium for use;

3. This reagent has patented technology that does not freeze when stored at -20 ¡æ, and does not require thawing when used. It can be taken out from -20 ¡æ and used immediately;

4. In order to maximize the efficacy of the drug, it is recommended to prepare and use the drug containing culture medium immediately. If the drug containing culture medium is not used up, it should be stored in a refrigerator at 4 ¡æ away from lightSave and use within 2 weeks. Preheat to 37 ¡æ before using the culture medium;

5. If individual cells are sensitive to this reagent and their growth rate is significantly affected, it is recommended to reduce the use or conduct dilution testing;

6. After treatment with highly efficient cell pollution removers, there will be good prevention and removal effects. However, if there are still issues with the environment, consumables, and reagents.There are pollution sources present, and cells may be contaminated again, so appropriate preventive measures need to be taken;

7. When adding this product for cell contamination prevention and removal, there is no need to add dual antibodies (penicillin streptomycin);

8. For your safety and health, please wear lab coats and disposable gloves when operating;

9. This product is for research or further production use only and should not be used for diagnosis or treatment.

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