Product Introduction
Interleukin-1 beta (IL-1¦Â) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, and hematopoietic activities, and plays a central role in the regulation of immune responses. Both IL-1¦Á and IL-1¦Â bind to the same receptor and have similar but not identical biological properties. Mature human IL-1¦Â shares 96% amino acid sequence identity with rhesus and 67%-78% with canine, mouse and rat IL-1¦Â.

Product Features
1. High Purity: >97% by SDS-PAGE and HPLC analyses.
2. High Biological Activity: ED50 <1.0 pg/mL as determined by a cell proliferation assay using murine D10S cells, corresponding to a specific activity of >1.0 ¡Á 10⁹ IU/mg.
3. E. coli Expression: Produced in Escherichia coli as a single non-glycosylated polypeptide chain containing 153 amino acids.
4. Low Endotoxin: <1 EU/µg as determined by LAL method.
5. Native Sequence: Full-length mature human IL-1¦Â with complete biological function.

Specifications
Size: Available in 2 µg, 10 µg, 100 µg, and 500 µg
Source: Escherichia coli
Molecular Weight: Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids
Amino Acid Sequence: APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIP VAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQ FPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS
Purity: >97% by SDS-PAGE and HPLC
Biological Activity: ED50 <1.0 pg/mL (cell proliferation assay using murine D10S cells), specific activity >1.0 ¡Á 10⁹ IU/mg
Endotoxin: <1 EU/µg (LAL method)
Physical Appearance: Sterile filtered white lyophilized powder
Formulation: Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4

Storage and Stability
Storage Conditions: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Shipping: The product is shipped at ambient temperature. Upon receipt, store immediately at the recommended temperature.
Shelf Life:
- 12 months from date of receipt, -20¡ãC to -70¡ãC as supplied.
- 1 month, 2¡ãC to 8¡ãC under sterile conditions after reconstitution.
- 3 months, -20¡ãC to -70¡ãC under sterile conditions after reconstitution.

Protocol (For Reference Only)
Important: Centrifuge the vial briefly before opening to bring the contents to the bottom. Reconstitute under aseptic conditions.
1. Reconstitution: Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL.
2. Aliquoting: Apportion stock solutions into working aliquots and store at ¡Ü -20¡ãC.
3. Further Dilution: Further dilutions should be made in appropriately buffered solutions.
4. Activity Assay: The biological activity can be measured by cell proliferation assay using murine D10S cells. The ED50 is less than 1.0 pg/mL.
5. Working Concentration: Optimal working concentrations should be determined empirically for each cell type and experimental system.

Precautions
1. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
2. Reconstitute with carrier protein (0.1% BSA) to maintain protein stability.
3. Stock solutions should be apportioned into working aliquots to avoid repeated freeze-thaw cycles.
4. IL-1¦Â is a potent proinflammatory cytokine; handle with appropriate safety precautions.
5. For research use only. Not for use in diagnostic or therapeutic procedures.

FAQ (Simplified)
Q1: How should I reconstitute IL-1¦Â?
A1: Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. The addition of BSA as a carrier protein is recommended for stability.
Q2: What is the specific activity of this protein?
A2: The ED50 is less than 1.0 pg/mL as determined by murine D10S cell proliferation assay, corresponding to a specific activity of >1.0 ¡Á 10⁹ IU/mg.
Q3: How does this IL-1¦Â compare across species?
A3: Mature human IL-1¦Â shares 96% amino acid sequence identity with rhesus and 67%-78% with canine, mouse and rat IL-1¦Â.
Q4: What is the molecular structure of this protein?
A4: The protein is a single non-glycosylated polypeptide chain containing 153 amino acids with an apparent molecular mass of approximately 17.3 kDa.

Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of working concentrations is recommended for specific cell types and experimental systems.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages, including loss of samples or data.
4. Wear appropriate protective clothing and gloves when handling this product.

Ordering Information
Catalog Number: CYT-P01584
Product Name: Recombinant Human Interleukin-1 beta
Size: 2 µg / 10 µg / 100 µg / 500 µg
Suggested Retail Price:
2 µg: CNY ¥1100.00 / USD $110.00 / EUR €132.00 / JPY ¥19800.00
10 µg: CNY ¥2560.00 / USD $256.00 / EUR €307.20 / JPY ¥46080.00
100 µg: CNY ¥10980.00 / USD $1098.00 / EUR €1317.60 / JPY ¥197640.00
500 µg: CNY ¥24630.00 / USD $2463.00 / EUR €2955.60 / JPY ¥443340.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.