T98G(ICGE-T98G,IC-84899)
Overview
Organism | Homo sapiens, human |
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Tissue |
brain
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Product Format | frozen |
Morphology | fibroblast |
Culture Properties | adherent |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease | glioblastoma multiforme |
Age | 61 years |
Gender | male |
Ethnicity | Caucasian |
Applications |
This cell line is a suitable transfection host.
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Storage Conditions | liquid nitrogen vapor phase |
Properties
Karyotype | This is a human cell line with hyperpentaploid chromosome count. The modal chromosome number should be around 128 to 132. The rate of cells with higher ploidies was 1.39%. Fourteen to 16 marker chromosomes were common to most cells. They were: der(1)t(1;?) (p36;?), i(6p), der(10)t(10;?) (q24;?), der (19)t(19;?) (q13;?), der(15)t(15;?) (q26?;?), minute metacentric and eight to ten others. Most of these structurally altered markers had complex interchromosomal exchanges. The der(10) and der(19) could be formed from a balanced translocation, i.e., t(10;19) (q24;q13). These two markers and the minute metacentric were present in three or more copies in most cells. There were six or more copies for N5, N7, N11, N13, N20, N21, and N22 in most cells. The X and N15 had only one copy. |
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Clinical Data |
61 years
Caucasian
male
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Tumorigenic | No |
Effects |
No, not tumorigenic in nude mice
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Comments |
When deprived of serum or when crowded, the cells enter a viable G1 arrested state.
The cells are anchorage independent.
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Background
Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle''s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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Subculturing |
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
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Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
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Culture Conditions |
Temperature: 37¡ãC
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