REH(ICGE-REH,IC-82964)
Overview
Organism | Homo sapiens, human |
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Product Format | frozen |
Morphology | lymphoblast |
Culture Properties | suspension |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease | acute lymphocytic leukemia (non-T; non-B) |
Storage Conditions | Liquid nitrogen vapor phase |
Disclosure | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
Properties
Antigen Expression |
CD3 A (17%) B (17%) C (20%), CD4 (15%), CD10 (55%)
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Genes Expressed |
CD3 A (17%) B (17%) C (20%), CD4 (15%), CD10 (55%)
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Background
Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
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Cryopreservation |
Freeze Medium: 95% (v/v) FBS; 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
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Culture Conditions |
Temperature: 37¡ãC
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