A673(ICGE-A673,IC-35423)
Catalog No.
Size
Price
IC-35423
10^6
$498.00
IC-35423
10^6
�547.80
Overview
| Organism | Homo sapiens, human |
|---|---|
| Tissue | muscle |
| Product Format | frozen |
| Morphology | polygonal |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Ewing''s Sarcoma |
| Age | 15 years |
| Gender | female |
| Shipping Information | frozen |
| Storage Conditions | liquid nitrogen vapor temperature |
Properties
| Clinical Data |
female
15 years
|
|---|---|
| Tumorigenic | Yes |
| Effects |
Yes, in immunosuppressed mice
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco''s Modified Eagle''s Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing |
Remove medium, rinse monolayer with fresh 0.25% trypsin, 0.03% EDTA solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:20 is recommended
Medium Renewal: 2 to 3 times per week
|
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
|
| Culture Conditions |
Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
