Calu-1(ICGE-Calu-1IC-39665)
Catalog No.
Size
Price
IC-39665
10^6
$467.00
IC-39665
10^6
�513.70
Overview
| Permits and Restrictions | |
|---|---|
| Organism | Homo sapiens, human |
| Tissue | lung; derived from metastatic site: pleura |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | grade III,epidermoid carcinoma |
| Age | 47 years |
| Gender | male |
| Ethnicity | Caucasian |
| Applications |
This cell line is a suitable transfection host.
|
| Storage Conditions | liquid nitrogen vapor phase |
Properties
| Karyotype | The stemline chromosome number is hypotriploid and the 2S component occurred at 14.2%. Modal chromosome number is 62. Seven markers occurred frequently, M1 (two copies per cell), M6 and M7 were found in most cells; M2 and M3, and M4 and M5 appeared to be mutually exclusive, i.e., only one of M2 or M3, and one of M4 or M5 were present in each cell. A Y chromosome was not detected by QM band examination, although the cell line was initiated from a male. |
|---|---|
| Clinical Data |
47 years
Caucasian
male
|
| Antigen Expression |
Antigen expression: Blood Type A; Rh+; HLA A10, A11, B15, Bw35
|
| Tumorigenic | Yes |
| Effects |
Yes, in nude mice; forms epidermoid carcinomas
Yes, in steroid treated hamsters
|
| Comments |
Ultrastructural features include numerous microvilli, prominent RER, lysosomes, lipid inclusions, no virus particles.
Contains the ras (K-ras) oncogene.
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy''s 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Interval: every 6 to 8 days
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
|
| Cryopreservation |
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
|
| Culture Conditions |
Temperature: 37°C
|
