NCI-H1650(ICGE-NCI-H1650,IC-82481)
Catalog No.
Size
Price
IC-82481
10^6
$467.00
IC-82481
10^6
�513.70
Overview
| Permits and Restrictions | |
|---|---|
| Organism | Homo sapiens, human |
| Tissue | lung; derived from metastatic site: pleural effusion |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | stage 3B,adenocarcinoma; bronchoalveolar carcinoma |
| Age | 27 years |
| Gender | male |
| Ethnicity | Caucasian, White |
| Storage Conditions | liquid nitrogen vapor phase |
Properties
Clinical Data
Caucasian, White
male
27 years
The patient was a smoker.
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. |
|---|---|
| Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
|
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% DMSO
Storage temperature: liquid nitrogen vapor phase
|
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
|
