NCI-H526(ICGE-NCI-H526,IC-82494)
Catalog No.
Size
Price
IC-82494
10^6
$523.00
IC-82494
10^6
�575.30
Overview
| Permits and Restrictions | |
|---|---|
| Organism | Homo sapiens, human |
| Tissue | lung; Derived from metastatic site: bone marrow |
| Cell Type | epithelial |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | round clusters in suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | stage E,carcinoma; variant small cell lung cancer |
| Age | 55 years adult |
| Gender | male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
Properties
| Derivation |
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a bone marrow metastasis taken from a patient prior to therapy.
|
|---|---|
| Clinical Data |
55 years adult
Caucasian
male
|
| Receptor Expression |
insulin-like growth factor II (IGF II); bombesin
|
| Oncogene | myc +; myb +; fes +; fms +; raf +; ras + |
| Tumorigenic | Yes |
| Effects |
Yes, the cells produce tumors in athymic nude mice
|
| Comments |
NCI-H526 expresses elevated levels of 2 biochemical markers of SCLC: neuron specific enolase and the brain isoenzyme of creatine kinase.
They do not express L-dopa carboxylase or bombesin-like immunoreactivity.
These cells express the c-kit gene as well as the N-myc gene, but not c-myc or L-myc.
N-myc is amplified and p75 c-myb expression was observed.
NCI-H526 also expresses the proto-oncogenes N-ras, Ki-ras, Ha-ras, and c-raf1.
Only trace amounts of the retinoblastoma susceptibility gene, RB mRNA were detected.
RB protein was not detected.
The cells express easily detectable levels of p53 mRNA compared to levels found in normal lung.
Abnormally sized mRNA was present.
The line has a reported colony-forming efficiency of 4.2% in soft agarose.
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. |
|---|---|
| Subculturing |
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases. Medium Renewal: Every 2 to 3 days
|
| Cryopreservation |
Culture medium, 95%; DMSO, 5%
|
| Culture Conditions |
Temperature: 37°C
|
