MEWO(ICGE-MEWO,IC-62761)
Catalog No.
Size
Price
IC-62761
10^6
$523.00
IC-62761
10^6
�575.30
Overview
| Organism | Homo sapiens, human |
|---|---|
| Tissue | skin, Derived from metastatic site: lymph node |
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | malignant melanoma |
| Age | 78 years |
| Gender | male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
Properties
| Derivation |
The MeWo cell line was initiated by Y. Kodera and M. Bean in 1974 from lymph node tissue.
Derived from metastatic site, lymph node
|
|---|---|
| Clinical Data |
78 years
male
White
|
| Antigen Expression |
Antigen expression: Blood type A; HLA A2, A26, Bw16, 18
|
| Genes Expressed |
Blood type A; HLA A2, A26, Bw16, 18
|
| Tumorigenic | Yes |
| Effects |
Yes, forms tumors in nude mice
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle''s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
culture medium 95%; DMSO, 5%
|
| Culture Conditions |
Temperature: 37°C
|
