U20S(ICGE-U20S,IC-85391)
Catalog No.
Size
Price
IC-85391
10^6
$498.00
IC-85391
10^6
�547.80
Overview
| Organism | Homo sapiens, human |
|---|---|
| Tissue | bone |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | osteosarcoma |
| Age | 15 years |
| Gender | female |
| Ethnicity | Caucasian |
| Applications |
This cell line is a suitable transfection host.
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Properties
| Karyotype | Cell line U-2 OS is chromosomally highly altered, with chromosome counts in the hypertriploid range. We did not find the hypodiploid cell population described by J. Ponten, et al., Instead, most of the population has slightly higher counts than first described. Very few normal chromosomes are present, but a high number of stable marker chromosomes are identified., Different chromosomal rearrangements involving the same chromosomes (N1, N7, N9, and N11 particularly), are seen. Twenty-two markers are found including: t(9qter--->9q21::1p36--->1p::?), 7p+, iso(17q), t(15q;?), 4q+, del(3)(q21), 5q(aberrant) and others. Ref |
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| Derivation |
J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.
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| Clinical Data |
15 years
Caucasian
female
J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.
|
| Antigen Expression |
Antigen expression: Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-)
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| Receptor Expression |
Receptor expression: insulin-like growth factor I (IGF-I); insulin-like growth factor II (IGF II)
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| Genes Expressed |
osteosacoma derived growth factor (ODGF),Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-)
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| Comments |
Viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses.
Mycoplasma contamination was detected and eliminated in 1972.
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Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy''''''''s 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Culture medium, 95%; DMSO, 5%
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| Culture Conditions |
Temperature: 37°C
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