Jurkat（clone E6-1）(ICGE-Jurkat（clone E6-1）,IC-676)
Catalog No.
Size
Price
IC-676
10^6
$546.00
IC-676
10^6
�600.60
Overview
| Organism | Homo sapiens, human |
|---|---|
| Tissue | peripheral blood |
| Cell Type | T lymphocyte |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | acute T cell leukemia |
| Gender | male |
| Applications |
This cell line is a suitable transfection host.
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| Storage Conditions | liquid nitrogen vapor phase |
Properties
| Karyotype | This is a pseudodiploid human cell line. The modal chromosome number is 46, occurring in 74% with polyploidy at 5.3%. The karyotype is 46,XY,-2,-18,del(2) (p21p23),del(18) (p11.2). Most cells had normal X and Y chromosomes. |
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| Derivation |
The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM.
The line was cloned from cells obtained from Dr. Kendall Smith and are mycoplasma free.
This is a clone of the Jurkat-FHCRC cell line, a derivative of the Jurkat cell line.
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| Clinical Data |
male
The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al.
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| Antigen Expression |
CD3; Homo sapiens, expressed
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| Receptor Expression |
Receptor expression: T cell antigen receptor, expressed |
| Genes Expressed |
interleukin-2 (interleukin 2, IL-2),CD3; Homo sapiens, expressed
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| Comments |
Clone E6-1 cells produce large amounts of IL-2 after stimulation with phorbol esters and either lectins or monoclonal antibodies against the T3 antigen (both types of stimulants are needed to induce IL-2 production.
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Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. |
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| Subculturing |
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Do not allow the cell density to exceed 3 X 106 cells/mL. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Interval: Maintain cultures at a cell concentraion between between 1 x 105 and 1 X 106 viable cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
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| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
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| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
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