RAJI(ICGE-RAJI,IC-60524)
Catalog No.
Size
Price
IC-60524
10^6
$620.00
IC-60524
10^6
�682.00
Overview
| Organism | Homo sapiens, human |
|---|---|
| Tissue | lymphoblast |
| Cell Type | B lymphocyte |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level |
2 [Cells contain Herpesvirus (EBV)]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Burkitt''s lymphoma |
| Age | 11 years |
| Gender | male |
| Ethnicity | Black |
| Applications |
This cell line is a suitable transfection host.
|
| Storage Conditions | liquid nitrogen vapor phase |
Properties
| Karyotype | Karyotype 100% stable within male diploid stemline of 46. Cells with 47 chromosomes frequently contained an extra "E" group chromosome.; There is 6% polyploidy and occasional disparity in the size of the homologs of the number 1 chromosome and the number 4 chromosome. |
|---|---|
| Derivation |
The Raji line of lymphoblast-like cells was established by R.J.V. Pulvertaft in 1963 from a Burkitt''s lymphoma of the left maxilla of an 11-year-old Black male.
|
| Clinical Data |
11 years
Black
male
The Raji line of lymphoblast-like cells was established by R.J.V. Pulvertaft in 1963 from a Burkitt''s lymphoma of the left maxilla of an 11-year-old Black male.
|
| Comments |
These cells are EBNA positive. The cells are partially resistant to poliovirus and vesicular stomatitis viruses. ATCC confirmed this line tested positive for the presence of Epstein Barr virus (EBV) viral DNA sequences via PCR. |
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. |
|---|---|
| Subculturing |
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively the cells may be collected by centrifugation. Cultures can then be established by resuspending the cells in fresh medium at 4 x 105 viable cells/mL. A maximum of 3 X 106 viable cells/mL is obtainable. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Medium Renewal: Every 2 to 3 days
|
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
|
| Culture Conditions |
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
