Human Cell Lines-Cell Lines-InCellGene LLC.-CellLines,Biomedicine reasearch Product,Cell Biology,Cell Culture,293T Cell,Media

Organism	Homo sapiens, human
Tissue	lung
Cell Type	fibroblast
Product Format	frozen
Morphology	fibroblast
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	Normal
Age	14 weeks gestation
Gender	Male
Ethnicity	Caucasian
Applications	efficacy testing transfection host viruscide testing
Storage Conditions	liquid nitrogen vapor phase

Karyotype	Chromosome Frequency Distribution 50 Cells: 2n = 46. This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 70% of cells. The rate of polyploidy was 3.6%. Both X and Y chromosomes were normal. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.

Derivation	The MRC-5 cell line was derived from normal lung tissue of a 14-week-old male fetus by J.P. Jacobs in September of 1966.
Clinical Data	Caucasian male 14 weeks gestation
Virus Susceptibility	Human poliovirus 1 Herpes simplex virus Vesicular stomatitis, Glasgow (Indiana) Vesicular stomatitis, Orsay (Indiana)
Comments	The cells are capable of 42 to 46 population doublings before the onset of senescence.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Eagle''''''''s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: 1 to 2 times per week
Cryopreservation	Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temerpature
Culture Conditions	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5%