HCT8(ICGE-HCT8,IC-60911)
Overview
Organism | Homo sapiens, human |
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Tissue | colon |
Product Format | frozen |
Morphology | epithelial |
Culture Properties | adherent |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease | ileocecal colorectal adenocarcinoma |
Age | 67 years adult |
Gender | male |
Storage Conditions | liquid nitrogen vapor phase |
Properties
Clinical Data
male
Genes Expressed
carcinoembryonic antigen (CEA) 0.5 ng/106 cells/10 days; alkaline phosphatase. The cells are positive for keratin by immunoperoxidase staining.
Cellular Products
carcinoembryonic antigen (CEA) 0.5 ng/10 exp6 cells/10 days; alkaline phosphatase; keratin
TumorigenicYesEffects
Yes, in nude mice
Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells
Comments
The HCT-8 line is identical to the HRT-18 cell line.
The cells are positive for keratin by immunoperoxidase staining.
Background
Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%. |
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Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
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Cryopreservation |
Freeze medium: complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
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Culture Conditions |
Temperature: 37¡ãC
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