HEC-1-A(ICGE-HEC-1-A,IC-61287)
Catalog No.
Size
Price
IC-61287
10^6
$498.00
IC-61287
10^6
�547.80
Overview
| Organism | Homo sapiens, human |
|---|---|
| Tissue | uterus; endometrium |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | adenocarcinoma |
| Age | 71 years |
| Gender | female |
Properties
| Karyotype | hypodiploid to hyperdiploid, modal number = 47 with large metacentric marker |
|---|---|
| Derivation |
This line and a substrain HEC-1-B (ATCC HTB-113) were isolated in 1968 by H. Kuramoto and associates from a patient with stage IA endometrial cancer.
|
| Clinical Data |
female
|
| Antigen Expression |
Blood Type B; Rh+
|
| Receptor Expression |
Receptor expression: platelet activating factor (PAF)
|
| Oncogene | c-fos + |
| Genes Expressed |
Oncogenes: c-fos +
|
| Tumorigenic | Yes |
| Effects |
Yes, in nude mice
(The cells form moderately well differentiated adenocarcinomas consistent with endometrial carcinoma (grade II).)
Yes, in the cheek pouch of cortisone treated hamsters
(The cells form typical papillary adenomas.)
|
| Comments |
PAF induces increased expression of c-fos.
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy''''''''s 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended |
| Cryopreservation |
Culture medium, 95%; DMSO, 5%
|
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
|
