Human Cell Lines-Cell Lines-InCellGene LLC.-CellLines,Biomedicine reasearch Product,Cell Biology,Cell Culture,293T Cell,Media

Organism	Homo sapiens, human
Tissue	mammary gland/breast
Cell Type	epithelial
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	Carcinoma
Age	74 years adult
Gender	female
Ethnicity	Caucasian
Storage Conditions	liquid nitrogen vapor phase

Karyotype	This is a hypotriploid human cell line with a modal chromosome number of 59. QM staining verified the absence of a Y chromosome. The rate of polyploidy in excess of the modal number is 33.8%. There were 8 consistent derivative chromosomes: del(1)(q12), del(2)(?q36), der(3)t(3;15)(q10;p10), der(5)t(5;8)(p10;q10), i(6)(p10), del(11)(p12),i(17)(q10), der(19)(19pter<-q13::5q13<-qter) plus two markers of unknown origin and one minute chromosome. Normal chromosome 17''''''''s were absent and only a single normal 15 was seen in most cells. No brightly fluorescent Y chromosomes were detected with QM staining. Number of cells examined = 50; Modal Chromosome Number = 59 with a range of 50 to 77; Polyploidy Rate = 33.8% Composite karyotype: 50-77 <3n> X, -1, del(1)(q12), -2, del(2)(?q36), der(3)t(3;15)(q10;p10),-4, -5,der(5)t(5;8)(p10;q10),-6, i(6)(p10), +8, -9, -10, -11, del(11)(p12), -12, -13, -14, -15, -15, -16, -17, -17, -17, i(17)(q10), -18, -19,der(19)(19pter<-q13::5q13<-qter), +22, +3 mar[cp12]. There are 8 consistent derivative chromosomes: del(1)(q12), del(2)(?q36), der(3)t(3;15)(q10;p10), der(5)t(5;8)(p10;q10), i(6)(p10), del(11)(p12), i(17)(q10), der(19)(19pter<-q13::5q13<-qter) plus two markers of unknown origin and one minute chromosome. Four other markers, including two derivative chromosome 1s were noted are lower frequency.
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Derivation	It was originated by A.J. Hackett, et al. along with the Hs 578Bst (see ATCC HTB-125), which is a normal fibroblast-like line from the same patient. The Hs 578T cell strain was derived from a carcinoma of the breast.
Clinical Data	74 years adult along with the Hs 578Bst (see ATCC HTB-125), which is a normal fibroblast-like line from the same patient. Caucasian female
Receptor Expression	Receptor expression: estrogen receptor, not expressed Ref
Tumorigenic	No
Effects	No, in immunosuppressed mice Yes, in semisolid medium
Comments	The Hs 578T line had a mixed polygonal morphology initially, but a stellate cell type was selected for during passage and by cloning. Aggregates of casein protein granules, desmosomes, tight junctions, lipid droplets and vesicularized smooth endoplasmic reticulum were observed by electron microscopy. As with Hs 578Bst, no estrogen receptors or endogenous viruses were detected.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco''''''''s Modified Eagle''''''''s Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml bovine insulin; fetal bovine serum to a final concentration of 10%.
Subculturing	Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C