WRL68(ICGE-WRL68,IC-61222)
Catalog No.
Size
Price
IC-61222
10^6
$498.00
IC-61222
10^6
�547.80
Overview
| Organism | Homo sapiens, human |
|---|---|
| Product Format | frozen |
| Culture Properties | adherent |
| Biosafety Level |
2 Cells contain Human Papilloma Viral (HPV) DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disclosure | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
Properties
| Comments |
A culture submitted to the ATCC in July of 1969 was found to be contaminated with mycoplasma, and was cured by treatment with Ciprofloxacin
|
|---|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle''s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
|
| Cryopreservation |
culture medium 95%; DMSO, 5%
|
| Culture Conditions |
Temperature: 37.0°C
|
