Human Cell Lines-Cell Lines-InCellGene LLC.-CellLines,Biomedicine reasearch Product,Cell Biology,Cell Culture,293T Cell,Media

Permits and Restrictions	View Restrictions
Organism	Homo sapiens, human
Tissue	pancreas; derived from metastatic site: liver
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	adenocarcinoma
Age	40 years
Gender	male
Ethnicity	Caucasian
Applications	This cell line is a suitable transfection host.

Karyotype	(P7) hypotriploid with abnormalities including dicentrics, breaks, acrocentric fragments, large submetacentric and subtelocentric chromosomes plus minute marker
Clinical Data	male 40 years Caucasian
Antigen Expression	Blood Type A; Rh+; HLA A2, A9, B13, B17
Receptor Expression	Progesterone receptor, positiveRef, estrogen receptor, alpha, positive Ref
Genes Expressed	mucin, Blood Type A; Rh+; HLA A2, A9, B13, B17
Cellular Products	mucin
Tumorigenic	Yes
Effects	Yes, in nude mice; forms adenocarcinoma consistent with pancreatic duct carcinoma
Comments	The cells will slough off of the growth surface if they become too heavy. Capan-1 expresses the cystic fibrosis transmembrane conductance regulator (CFTR) and secrete gastric type mucins.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Iscove''s Modified Dulbecco''s Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing	Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco''s phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation	culture medium 95%; DMSO, 5%
Culture Conditions	Temperature: 37°C