A-498(ICGE-A-498,IC-45551)
Overview
Organism | Homo sapiens, human |
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Tissue | kidney |
Product Format | frozen |
Morphology | epithelial |
Culture Properties | adherent |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease | carcinoma |
Age | 52 years |
Gender | female |
Storage Conditions | liquid nitrogen vapor phase |
Properties
Derivation |
S. Aaronson isolated this line using techniques as described for ATCC HTB-41.
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Clinical Data |
female
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Tumorigenic | Yes |
Effects |
Yes, in nude mice; forms undifferentiated carcinoma; also forms tumors in anti thymocyte serum treated newborn mice
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Comments |
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Background
Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle''s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Twice per week
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Cryopreservation |
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
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Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37¡ãC
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