G-401(ICGE-G-401,IC-81267)
Catalog No.
Size
Price
IC-81267
10^6
$489.00
IC-81267
10^6
�537.90
Overview
| Organism | Homo sapiens, human |
|---|---|
| Tissue |
kidney
|
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | rhabdoid tumor (formerly classified as Wilms'''''''' tumor) |
| Age | 3 months |
| Gender | male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor temperature |
Properties
| Karyotype | The cell line has the 46, XY karyotype. There are no apparent marker chromosomes. The rate of polyploidy was 2.8%. The presence of the Y chromosome was confirmed in Q-band observation. |
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| Derivation |
G-401 was deposited as a cell line derived from a Wilms'''''''' tumor.
|
| Clinical Data |
3 months
Caucasian
male
|
| Genes Expressed | nephroblast growth factor (NB-GF) |
| Cellular Products |
nephroblast growth factor (NB-GF)
|
| Tumorigenic | Yes |
| Effects |
Yes, forms colonies in soft agar
|
| Comments |
G-401 was deposited as a cell line derived from a Wilms'''''''' tumor. Due to a change in the classification of such tumors, the cell line was examined by Garvin et al. and found to be more appropriately classified as derived from a rhabdoid tumor of the kidney. Ref
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Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy''''''''s 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
|
| Cryopreservation |
Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor temperature
|
| Culture Conditions |
Temperature: 37°C
|
