Human Cell Lines-Cell Lines-InCellGene LLC.-CellLines,Biomedicine reasearch Product,Cell Biology,Cell Culture,293T Cell,Media

Permits and Restrictions	View Restrictions
Organism	Homo sapiens, human
Tissue	brain; derived from metastatic site: supra-orbital area
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	neuroepithelioma
Age	14 years
Gender	female
Ethnicity	Caucasian
Applications	This cell line is a suitable transfection host.
Storage Conditions	liquid nitrogen vapor phase

Karyotype	The cell line is a pseudodiploid human female (XX), with chromosome counts in the diploid range and a modal chromosome number of 46. Normal chromosomes N3 and N10 are absent, and many (N1, N2, N4, N15, N16, N17, N21, and N22) are monosomic. Normal chromosome N8 is most often tetrasomic. The remainder of normal chromosomes were usually paired. Numerous marker chromosomes are present including: 1p+, der(3)t(2;3)(q24;q27), del(4)(p12), 11q+, del(2)(q23), ampl.(17)(p12), ampl.(16)(q13), del(15)(q13q22), 21p+, iso(3q), del(22)(q11q13). Marker chromosomes M2 and M3 appear to us to be identical to two markers (M4 and M3, respectively) described by R.C. Seeger, et al.for this cell line.Ref
Derivation	This is one of two cell lines (see ATCC HTB-11) of neurogenic origin derived by J.L. Biedler. SK-N-MC was isolated in September of l971.
Clinical Data	14 years Caucasian female
Antigen Expression	Antigen expression: Blood Type O; Rh+
Genes Expressed	Blood Type O; Rh+
Tumorigenic	Yes
Effects	Yes, in hamster cheek Yes, in nude mice
Comments	This cell line was found to have moderate dopamine - beta - hydroxylase activity as well as formaldehyde induced fluorescence indicative of intracellular catecholamines.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Eagle''s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C