Human Cell Lines-Cell Lines-InCellGene LLC.-CellLines,Biomedicine reasearch Product,Cell Biology,Cell Culture,293T Cell,Media

Permits and Restrictions	View Permits View Restrictions
Organism	Homo sapiens, human
Tissue	bone marrow
Product Format	frozen
Morphology	epithelial
Culture Properties	mixed, adherent and suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	neuroblastoma
Age	4 years
Gender	female
Applications	This cell line is a suitable transfection host.
Storage Conditions	liquid nitrogen vapor phase

Karyotype	modal number = 47; the cells possess a unique marker comprised of a chromosome 1 with a complex insertion of an additional copy of a 1q segment into the long arm, resulting in trisomy of 1q Ref

Derivation	SH-SY5Y is a thrice cloned (SK-N-SH -> SH-SY -> SH-SY5 -> SH-SY5Y) subline of the neuroblastoma cell line SK-N-SH (seeATCC HTB-11) which was established in 1970 from a metastatic bone tumor.
Clinical Data	4 years female
Antigen Expression	Blood Type A; Rh+
Comments	SH-SY5Y cells have a reported saturation density greater than 1 X 106 cells/cm2. They are reported to exhibit moderate levels of dopamine beta hydroxylase activity. Ref

Complete Growth Medium	The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagle''''''''s Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	These cells grow as a mixture of floating and adherent cells. The cells grow as clusters of neuroblastic cells with multiple, short, fine cell processes (neurites). Cells will aggregate, form clumps and float. Remove the medium with the floating cells, and recover the cells by centrifugation. Rinse the adherent cells with fresh 0.25% trypsin, 0.53 mM EDTA solution, add an additional 1 to 2 mL of trypsin solution, and let the culture sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate, combine with the floating cells recovered above and dispense into new flasks. For slightly adherent cells that you don’t want to stick, use Corning® T-75 Ultra Low Attachment Flasks (catalog # 3814) to subculture this product. Subcultivation Ratio: A subcultivation ratio of 1:20 to 1:50 is recommended Medium Renewal: Every 4 to 7 days
Cryopreservation	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C