SK-N-SH(ICGE-SK-N-SH,IC-83451)
Catalog No.
Size
Price
IC-83451
10^6
$489.00
IC-83451
10^6
�537.90
Overview
| Permits and Restrictions | |
|---|---|
| Organism | Homo sapiens, human |
| Tissue |
brain; derived from metastatic site: bone marrow
|
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | neuroblastoma |
| Age | 4 years |
| Gender | female |
| Applications |
SK-N-SH has been used as a target cell line in cell mediated cytotoxicity assays and is a suitable transfection host.
|
Properties
| Karyotype | The cell line is hyperdiploid human female (XX), with the modal chromosome number of 47. Normal chromosomes N9 and N22 are single. One copy of each of these chromosomes is structurally altered to form the two marker chromosomes 9q+ and 22q+., Chromosomes N7 is trisomic. Extra bands were found on one copy of chromosome N7, thereby forming a marker chromosome as described by R.C. Seeger. May have been translocated in part(s) to the q arms of chromosomes N9 and N22. |
|---|---|
|
|
|
| Derivation |
The SK-N-SH line was developed by J.L. Biedler and differs from SK-N-MC (see ATCC HTB-10) in that it exhibits a longer doubling time and higher levels of dopamine - beta - hydroxylase.
|
| Clinical Data |
female
|
| Antigen Expression | Blood Type A; Rh+ |
| Genes Expressed |
plasminogen activator,shows increased expression of M-CSF after treatment with amyloid-beta peptide.
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle''''''''s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 1 to 2 times per week
|
| Cryopreservation |
Culture medium, 95%; DMSO, 5%
|
| Culture Conditions |
Temperature: 37°C
|
