MDA-MB-436(ICGE-MDA-MB-436,IC-41033)
Catalog No.
Size
Price
IC-41033
10^6
$459.00
IC-41033
10^6
�504.90
Overview
| Permits and Restrictions | |
|---|---|
| Organism | Homo sapiens, human |
| Tissue |
mammary gland/breast; derived from metastatic site: pleural effusion
|
| Product Format | frozen |
| Morphology | pleomorphic with multinucleated component cells |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | adenocarcinoma |
| Age | 43 years |
| Gender | female |
| Ethnicity | Caucasian |
Properties
| Karyotype | modal number = 45 |
|---|---|
| Clinical Data |
43 years
Caucasian
female
|
| Genes Expressed |
tubulin; actin
|
| Cellular Products | Cellular products: tubulin, actin |
| Tumorigenic | No |
| Effects |
No, in immunosuppressed mice
Yes, in semisolid medium
|
| Comments |
The line is pleomorphic and most cells react intensely with anti tubulin antibody as demonstrated by indirect immunofluorescence staining.
|
Background
| Complete Growth Medium |
Leibovitz''s L-15 medium with 10 mcg/ml insulin, 16 mcg/ml glutathione, 90%; fetal bovine serum, 10%.
(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
|---|---|
| Subculturing |
Subcultures are prepared by scraping. Remove spent medium, add 3 to 5 ml of fresh medium, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
|
| Cryopreservation |
Culture medium, 95%; DMSO, 5%
|
| Culture Conditions |
Atmosphere: air, 100%
Temperature: 37°C |
