MCF-7(ICGE-MCF-7,IC-13097)
MCF-7(ICGE-MCF-7,IC-13097)
MCF-7(ICGE-MCF-7,IC-13097)
MCF-7(ICGE-MCF-7,IC-13097)
Catalog No.
Size
Price
IC-13097
10^6
$459.00
IC-13097
10^6
€504.90
Overview
Organism Homo sapiens, human
Tissue mammary gland, breast; derived from metastatic site: pleural effusion
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease adenocarcinoma
Age 69 years adult
Gender female
Ethnicity Caucasian
Applications
These cells are suitable as a transfection host.
Storage Conditions liquid nitrogen vapor phase
Properties
Karyotype modal number = 82; range = 66 to 87.
The stemline chromosome numbers ranged from hypertriploidy to hypotetraploidy, with the 2S component occurring at 1%. There were 29 to 34 marker chromosomes per S metaphase; 24 to 28 markers occurred in at least 30% of cells, and generally one large submetacentric (M1) and 3 large subtelocentric (M2, M3, and M4) markers were recognizable in over 80% of metaphases. No DM were detected. Chromosome 20 was nullisomic and X was disomic.


Antigen Expression Antigen expression: Blood Type O; Rh+
Receptor Expression Receptors expression: estrogen receptor, expressed
Oncogene The cells express the WNT7B oncogene. Ref
Genes Expressed insulin-like growth factor binding proteins (IGFBP) BP-2; BP-4; BP-5
Cellular Products
insulin-like growth factor binding proteins (IGFBP) BP-2; BP-4; BP-5
Comments
The MCF7 line retains several characteristics of differentiated mammary epithelium including ability to process estradiol via cytoplasmic estrogen receptors and the capability of forming domes. The cells express the WNT7B oncogene.
Growth of MCF7 cells is inhibited by tumor necrosis factor alpha (TNF alpha).
Secretion of IGFBP''''''''s can be modulated by treatment with anti-estrogens.
Background
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle''''''''s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml human recombinant insulin; fetal bovine serum to a final concentration of 10%. 
Subculturing

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.

Note: if floating cells are present, it is recommended that they be transferred at the first two (2) subcultures as described below. It is not necessary to transfer floating cells for subsequent subcultures.

  1. Remove culture medium to a centrifuge tube.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37¡ãC to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Transfer the cell suspension to the centrifuge tube with the medium and cells from step 1, and centrifuge at approximately 125 xg for 5 to 10 minutes. Discard the supernatant.
  6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  7. Incubate cultures at 37¡ãC.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37¡ãC
InCellGene


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