JEG-3(ICGE-JEG-3,IC-5530)
JEG-3(ICGE-JEG-3,IC-5530)
JEG-3(ICGE-JEG-3,IC-5530)
JEG-3(ICGE-JEG-3,IC-5530)
Catalog No.
Size
Price
IC-5530
10^6
$459.00
IC-5530
10^6
€504.90
Overview
Organism Homo sapiens, human
Tissue placenta
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease choriocarcinoma
Applications

This cell line is a suitable transfection host.

Storage Conditions liquid nitrogen vapor phase
Properties
Karyotype This is a hypertriploid human cell line. The modal chromosome number is 71, occurring at 34%, and polyploidy at 2.6%. The t(4;11)(p15;q13), i(13q), t(10p15q), del(18)(q21), and 6 other markers are common to most cells, and two other markers are found in some. Giant satellites are seen in one N14, and two N22. N2, N5, and N9 have 4 copies, and N7, N13, N18, N21 and X a single copy. A single Y chromosome is detected by Q-band examination.


Derivation
This is one of six clonally derived lines isolated from the Woods strain of the Erwin-Turner tumor by Kohler and associates.
Genes Expressed
human chorionic gonadotropin (hCG), human chorionic somatomammotropin (placental lactogen); progesterone
Tumorigenic Yes
Effects
Yes, in nude mice; forms malignant tumor consistent with choriocarcinoma
Comments
The cells are able to transform steroid precursors to estrone and estradiol.
Background
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle''''''''s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. 
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37¡ãC) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37¡ãC
InCellGene


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