LNCaP clone FGC(ICGE-LNCaP clone FGC,IC-17943)
LNCaP clone FGC(ICGE-LNCaP clone FGC,IC-17943)
LNCaP clone FGC(ICGE-LNCaP clone FGC,IC-17943)
LNCaP clone FGC(ICGE-LNCaP clone FGC,IC-17943)
Catalog No.
Size
Price
IC-17943
10^6
$459.00
IC-17943
10^6
€504.90
Overview
Organism Homo sapiens, human
Tissue prostate; derived from metastatic site: left supraclavicular lymph node
Product Format frozen
Morphology epithelial
Culture Properties adherent, single cells and loosely attached clusters
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease carcinoma
Age 50 years adult
Gender male
Ethnicity Caucasian
Applications
This cell line is suitable as a transfection host.
Storage Conditions liquid nitrogen vapor phase
Properties
Karyotype This is a hypotetraploid human cell line. The modal chromosome number was 84, occurring in 22% of cells. However, cells with chromosome counts of 86 (20%) and 87 (18%) also occurred at high frequencies. The rate of cells with higher ploidies was 6.0%.


Derivation
LNCaP clone FGC was isolated in 1977 by J.S. Horoszewicz, et al., from a needle aspiration biopsy of the left supraclavicular lymph node of a 50-year-old Caucasian male (blood type B+) with confirmed diagnosis of metastatic prostate carcinoma.
Clinical Data
50 years adult
from a needle aspiration biopsy of the left supraclavicular lymph node of a 50-year-old Caucasian male (blood type B+) with confirmed diagnosis of metastatic prostate carcinoma.
Caucasian
male
Receptor Expression
androgen receptor, positive; estrogen receptor, positive
Genes Expressed
human prostatic acid phosphatase; prostate specific antigen
Cellular Products
human prostatic acid phosphatase; prostate specific antigen
Tumorigenic Yes
Effects
Yes, in soft agar
Yes, the cells are tumorigenic in nude mice
Comments
These cells are responsive to 5-alpha-dihydrotestosterone (growth modulation and acid phosphatase production).
The cells do not produce a uniform monolayer, but grow in clusters which should be broken apart by repeated pipetting when subcultures are prepared.
They attach only lightly to the substrate, do not become confluent and rapidly acidify the medium.
Growth is very slow.
The cells should be allowed to incubate undisturbed for the first 48 hours after subculture.
When flask cultures are shipped, the majority of the cells become detached from the flask and float in the medium. Upon receipt, incubate the flask (in the usual position for monolayer cultures) for 24 to 48 hours to allow the cells to re-attach. The medium can then be removed and replaced with fresh medium.
If desired, the contents of the flask can be collected, centrifuged at 300 X g for 15 minutes, resuspended in 10 mL of medium and dispensed into a single flask.
Background
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. 
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37¡ãC to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels. 
    Maintain cultures at a cell concentration between 1 X 104and 2 X 105 cells/cm2.
  6. Incubate cultures at 37¡ãC.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37¡ãC
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