MRC-5(ICGE-MRC-5,IC-82237)
MRC-5(ICGE-MRC-5,IC-82237)
MRC-5(ICGE-MRC-5,IC-82237)
MRC-5(ICGE-MRC-5,IC-82237)
Catalog No.
Size
Price
IC-82237
10^6
$512.00
IC-82237
10^6
€563.20
Overview
Organism Homo sapiens, human
Tissue lung
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Normal
Age 14 weeks gestation
Gender Male
Ethnicity Caucasian
Applications
efficacy testing
transfection host
viruscide testing
Storage Conditions liquid nitrogen vapor phase
Properties
Karyotype Chromosome Frequency Distribution 50 Cells: 2n = 46. This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 70% of cells. The rate of polyploidy was 3.6%. Both X and Y chromosomes were normal. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.


Derivation
The MRC-5 cell line was derived from normal lung tissue of a 14-week-old male fetus by J.P. Jacobs in September of 1966.
Clinical Data Caucasian
male
14 weeks gestation
Virus Susceptibility Human poliovirus 1 
Herpes simplex virus 
Vesicular stomatitis, Glasgow (Indiana) 
Vesicular stomatitis, Orsay (Indiana) 
Comments
The cells are capable of 42 to 46 population doublings before the onset of senescence.
Background
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle''''''''s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. 
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37¡ãC to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37¡ãC.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 1 to 2 times per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temerpature
Culture Conditions
Temperature: 37¡ãC 
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
InCellGene


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