RT4(ICGE-RT4,IC-82968)
Catalog No.
Size
Price
IC-82968
10^6
$512.00
IC-82968
10^6
�563.20
Overview
| Organism | Homo sapiens, human |
|---|---|
| Tissue |
urinary bladder
|
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | transitional cell papilloma |
| Age | 63 years |
| Gender | male |
| Ethnicity | Caucasian |
| Applications |
This cell line is a transfection host.
|
| Storage Conditions | liquid nitrogen vapor phase |
Properties
| Karyotype | modal number = 49; range = 42 to 55. The cell line is aneuploid human male, with chromosome counts in the near-diploid range. However, the near-tetraploid population may become predominant within very few passages. The 48 and 49 chromosome karyotypes had a single X and a single Y chromosome. Three of the karyotypes at higher ploidy had two X chromosomes and a single Y chromosome. Four marker chromosomes were identified: del(6)(q21), del(10)(p11), 14p+, 12p+. Marker chromosome M4 was not seen in the karyotypes prepared from higher ploidy metaphases. |
|---|---|
| Clinical Data |
63 years
Caucasian
male
|
| Antigen Expression | Antigen expression: HLA A25(10), A3, B12, Cw3; Blood Type O |
| Tumorigenic | Yes |
| Effects |
Yes, in cheek pouch of steroid-treated hamsters
|
| Comments |
A contamination with Mycoplasma orale was cured in October 1971.
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy''s 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
|
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
|
| Culture Conditions |
Temperature: 37°C
|
