T24(ICGE-T24,IC-84658)
Catalog No.
Size
Price
IC-84658
10^6
$512.00
IC-84658
10^6
�563.20
Overview
| Organism | Homo sapiens, human |
|---|---|
| Tissue | urinary bladder |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | transitional cell carcinoma |
| Age | 81 years |
| Gender | female |
| Ethnicity | Caucasian |
| Applications |
This cell line is a suitable transfection host.
|
Properties
| Karyotype | hypodiploidy to hypopentaploidy; stemline 86; 2 to 4 telocentrics; 3 to 4 minutes, hypotetraploid to hypertetraploid with abnormalities including dicentrics, breaks, pulverization, minutes and telocentric markers |
|---|---|
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| Clinical Data |
Leukocytes and sera from patients with transitional cell carcinoma were cytotoxic to T24 and related lines.
81 years
Caucasian
female
|
| Antigen Expression |
HLA A1, A3, B18, Bw35, Cw4, DRw2, Dw4
|
| Genes Expressed |
tumor specific antigen, HLA A1, A3, B18, Bw35, Cw4, DRw2, Dw4
|
| Cellular Products |
tumor specific antigen
|
| Tumorigenic | Yes |
| Effects |
Yes, in hamster cheek pouch
No, in nude mice
|
| Comments |
Leukocytes and sera from patients with transitional cell carcinoma were cytotoxic to T24 and related lines.
Cells have a 19 hour generation time.
Contains the ras (H-ras) oncogene.
|
Background
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy''''''''s 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
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| Cryopreservation |
Culture medium, 95%; DMSO, 5%
|
| Culture Conditions |
Temperature: 37°C
|
