Human Cell Lines-Cell Lines-InCellGene LLC.-CellLines,Biomedicine reasearch Product,Cell Biology,Cell Culture,293T Cell,Media

Organism	Homo sapiens, human
Tissue	brain
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	Likely glioblastoma
Age	unknown
Gender	male
Ethnicity	unknown
Applications	This cell line is a suitable transfection host.
Storage Conditions	liquid nitrogen vapor phase

Karyotype	This is a hypodiploid human cell line with the modal chromosome number of 44 occurring in 48% of cells. The rate of higher ploidy was 5.9%., Twelve markers were common to all cells, including der(1)t(1;3) (p22;q21), der(16)t(1;16) (p22;p12), del(9) (p13) and nine others. The marker der(1) had two copies in most cells. There was only one copy of normal X. N1, N6 and N9 were not found.

Derivation	This is one of a number of cell lines derived from malignant gliomas (see also ATCC® HTB-15™ and HTB-16™) by J. Ponten and associates from 1966 to 1969 (see also Allen, 2016).
Clinical Data	male
Tumorigenic	Yes
Effects	Yes, in nude mice inoculated subcutaneously with 10(7) cells
Comments	Mycoplasma contamination was eliminated in September 1975. The ATCC® HTB-14™ cell line was deposited at ATCC in 1982. STR profiling, Y-chromosome paint, and Q-band assay confirmed that the cell line is male in origin. Based on current literature, the cell line is likely a glioblastoma of CNS origin (Allen, 2016).

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Eagle''''''''s Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco''''''''s phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation	Culture medium, 95%; DMSO, 5%
Culture Conditions	Atmosphere: 5% CO2 in air recommended Temperature: 37°C