Human Cell Lines-Cell Lines-InCellGene LLC.-CellLines,Biomedicine reasearch Product,Cell Biology,Cell Culture,293T Cell,Media

Organism	Homo sapiens, human
Tissue	bone marrow; derived from metastatic site: pleural effusion
Product Format	frozen 1.0 mL
Morphology	hematopoietic
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	chronic myeloid leukemia at blast crisis
Age	53
Gender	female
Applications	This is a clone of the parental K562 cell line which is resistant to the tyrosine kinase inhibitor imatinib mesylate, and is paired to the K562-s clone which is sensitive to imatinib. The paired K562-s and K562-r clones are useful cell line models of sensitivity and resistance to a tyrosine kinase inhibitor used in the treatment of chronic myeloid leukemia.
Storage Conditions	liquid nitrogen vapor phase


Derivation	Erythroid-Myeloid precursor cell line derived from chronic myeloid leukemia (CML) in blast crisis.
Clinical Data	female 53 years
Oncogene	BCR-ABL of the e14a2 fusion transcript type
Comments	Oncogenes: BCR-ABL of the e14a2 fusion transcript type Erythroid-myeloid precursor cell line derived from chronic myeloid leukemia (CML) in blast crisis

Complete Growth Medium	The complete medium for this cell line is RPMI-1640 + 10% FBS + 2 mM L-Glutamine + 1 μM Imatinib Details for preparation of 500 ml of complete media Medium: 500 mL RPMI-1640 (ATCC 30-2001) Serum: 56 mL FBS (ATCC 30-2020) Additive: 5.6 mL L-Glutamine (200 mM, ATCC 30-2214) 0.5 mL 1 mM Imatinib working stock (see below) To prepare 1 mM Imatinib working stock, aseptically combine the following: 25 mg Imatinib (Sigma catalog # CDS022173-25MG) into 45 mL Culture grade water (Hyclone catalog # SH30529 Note: supplement Imatinib at each reseed rather than including in culture medium bottle. The shelf life for Imatinib in culture medium is unknown, so do not use a prepared culture medium bottle for more than 30 days. Additionally, if cell growth appears to slow during cell line expansion, prepare a new bottle of fresh medium.
Subculturing	Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2.5 to 3.5 X 105 cells/mL and maintain between 2 X 105 and 1 X 106 cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C

Complete Growth Medium

The complete medium for this cell line is RPMI-1640 + 10% FBS + 2 mM L-Glutamine + 1 μM Imatinib Details for preparation of 500 ml of complete media

Medium: 500 mL RPMI-1640 (ATCC 30-2001)
Serum: 56 mL FBS (ATCC 30-2020)
Additive: 5.6 mL L-Glutamine (200 mM, ATCC 30-2214)
0.5 mL 1 mM Imatinib working stock (see below)

To prepare 1 mM Imatinib working stock, aseptically combine the following:

25 mg Imatinib (Sigma catalog # CDS022173-25MG) into 45 mL Culture grade water (Hyclone catalog # SH30529
Note: supplement Imatinib at each reseed rather than including in culture medium bottle. The shelf life for Imatinib in culture medium is unknown, so do not use a prepared culture medium bottle for more than 30 days. Additionally, if cell growth appears to slow during cell line expansion, prepare a new bottle of fresh medium.

Subculturing

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2.5 to 3.5 X 105 cells/mL and maintain between 2 X 105 and 1 X 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37°C