Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

The ES-2 cell line was established from a surgical tumor specimen taken from a 47 year old black woman.

47 years

Black

female

P glycoprotein

P glycoprotein

Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells.

The tumor was described as a poorly differentiated ovarian clear cell carcinoma.

Initially, the cells were grown in soft agar.

The cells exhibit low to moderate resistance to a number of chemotherapeutic agents including doxorubicin, cisplatin, carmustine, etoposide and cyanomorpholinodoxorubicin (MRA-CN).

ES-2 cells express low levels of P glycoprotein.

Remove spent medium, add fresh EDTA solution (0.15 g disodium EDTA, 4.0 g NaCL, 0.28 g sodium bicarbonate, 0.5 g dextrose and 0.2 g KCl dissolved in 500 mL double distilled water). Allow the cells to sit at room temperature for a few minutes, and dislodge the cells by rapping the side of the flask sharply with the palm of your hand. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage Temperature: Liquid nitrogen vapor phase

Temperature: 37¡ãC