U-937(ICGE-U-937,IC-85625)
U-937(ICGE-U-937,IC-85625)
U-937(ICGE-U-937,IC-85625)
Catalog No.
Size
Price
IC-85625
10^6
$498.00
IC-85625
10^6
€547.80
Overview
Permits and Restrictions

View Restrictions

Organism Homo sapiens, human
Tissue Pleura/pleural effusion, lymphocyte, Myeloid
Product Format frozen
Morphology monocyte
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease histiocytic lymphoma
Age 37 years
Gender male
Ethnicity Caucasian
Applications
This cell line is suitable as a transfection host
Storage Conditions liquid nitrogen vapor phase
Properties
Images ATCC CRL-1593.2 Cell Micrograph
Derivation
The U-937 cell line was derived by Sundstrom and Nilsson in 1974 from malignant cells obtained from the pleural effusion of a patient with histiocytic lymphoma.
Clinical Data
37 years
Caucasian
male
The U-937 cell line was derived by Sundstrom and Nilsson in 1974 from malignant cells obtained from the pleural effusion of a patient with histiocytic lymphoma.
Receptor Expression
complement (C3)
Genes Expressed
lysozyme; beta-2-microglobulin (beta 2 microglobulin); tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid (PMA)
Cellular Products
lysozyme; beta-2-microglobulin (beta 2 microglobulin); tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid (PMA)
Comments
Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures,
The cells are negative for immunoglobulin production and Epstein-Barr virus expression.
The cells express the Fas antigen, and are sensitive to TNF and anti-Fas antibodies.
In 1994, PCR and cytogenetic analyses showed that a number of stocks of U-937 were contaminated with the human myeloid leukemia cell line, K-562.
In the earliest stocks available, the level of contamination was 0.6%.
Distribution was discontinued in March 1994, except if required for patent purposes.
Anyone who wishes to receive a sample of this original material should contact the Head of the ATCC Patent Depository.
A stock of CRL-1593 found to be free of K-562 was propagated continuously for 8 weeks and tested weekly by PCR.
Distribution and seed stocks give DNA profiles characteristic of U-937 only.
Such preparations are now offered as authentic U-937 (ATCCCRL-1593.2) and are believed to be free of second subpopulations.
Background
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. 
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 105 viable cells/mL. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Interval: Maintain cell density between 1 X 105 and 2 X 106viable cells/mL.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37¡ãC
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