2-CTC Resin, 934816-82-7, IC-03151309
Product Introduction
2-CTC Resin (2-chlorotrityl chloride resin) is a highly acid-labile polymeric support widely used in solid-phase peptide synthesis (SPPS) and solid-phase organic synthesis. The resin consists of cross-linked polystyrene beads (100-200 mesh, 1% DVB) functionalized with 2-chlorotrityl chloride groups, which react readily with carboxylic acids, alcohols, and amines to form ester or amide linkages. The resulting 2-chlorotrityl linkages are exceptionally sensitive to mild acid treatment (e.g., 0.5-1% TFA in DCM), enabling the release of protected peptides and acid-sensitive products under conditions that preserve sensitive functional groups and avoid side-chain deprotection. This property makes 2-CTC Resin particularly valuable for the synthesis of protected peptide fragments, cyclic peptides, peptide acids, and combinatorial libraries. The resin loading range of 0.4-3.0 mmol/g provides flexibility for different synthesis scales and applications.
Product Features
1. Highly Acid-Labile Linkage: Cleavage under mild acid conditions (0.5-1% TFA) preserves sensitive functional groups and side-chain protecting groups.
2. Broad Application: Suitable for protected peptide fragment synthesis, cyclic peptides, peptide acids, and solid-phase organic synthesis.
3. Uniform Bead Size: 100-200 mesh beads ensure consistent swelling, reagent diffusion, and synthesis performance.
4. 1% DVB Cross-Linking: Provides appropriate mechanical stability and swelling characteristics in organic solvents.
5. Flexible Loading Range: 0.4-3.0 mmol/g loading accommodates different synthesis scales and application requirements.
Specifications
Size: 10 g / 50 g / 100 g
CAS Number: 934816-82-7
Mesh Size: 100-200 mesh
Cross-Linking: 1% DVB
Loading: 0.4-3.0 mmol/g
Appearance: Powder
Storage and Stability
Storage Conditions: Store powder at -20ˇăC for 3 years or at 4ˇăC for 2 years. For solutions: store at -80ˇăC for 6 months or -20ˇăC for 1 month.
Shelf Life: 3 years at -20ˇăC (powder); 2 years at 4ˇăC (powder); 6 months at -80ˇăC (solution); 1 month at -20ˇăC (solution).
Protocol (For Reference Only)
Important: Handle resin under anhydrous conditions to prevent hydrolysis of the chlorotrityl chloride groups. Use anhydrous solvents and inert atmosphere for coupling reactions.
1. Resin Swelling: Swell the resin in anhydrous DCM for 20-30 minutes before use.
2. First Amino Acid Loading: Dissolve the Fmoc-amino acid (1-1.5 equivalents relative to resin loading) in anhydrous DCM, add DIPEA (2-3 equivalents), and mix with the swollen resin. Incubate at room temperature for 1-2 hours with gentle agitation.
3. Capping: After loading, cap unreacted chlorotrityl groups with a solution of DCM/methanol/DIPEA (17:2:1) for 10-15 minutes.
4. Washing: Wash the resin thoroughly with DCM, DMF, and DCM to remove excess reagents.
5. Peptide Elongation: Perform Fmoc-based SPPS according to standard protocols.
6. Cleavage: Cleave the peptide from the resin using 0.5-1% TFA in DCM for 5-10 minutes, repeated 2-3 times. Neutralize the combined cleavage solutions with pyridine or DIPEA.
7. Analysis: Confirm the identity and purity of the cleaved product by HPLC and mass spectrometry.
Precautions
1. 2-CTC Resin is moisture-sensitive; store under anhydrous conditions and handle in a dry atmosphere.
2. Use anhydrous solvents (DCM, DMF) for all coupling and washing steps.
3. Chlorotrityl chloride resin may release HCl upon hydrolysis; handle in a well-ventilated area.
4. Dispose of resin waste according to institutional guidelines.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the advantage of 2-CTC Resin?
A1: The 2-chlorotrityl linkage is highly acid-labile, allowing cleavage under mild acid conditions (0.5-1% TFA) that preserve sensitive functional groups and side-chain protecting groups.
Q2: What is the recommended loading procedure?
A2: Dissolve the Fmoc-amino acid in anhydrous DCM with DIPEA and mix with the swollen resin for 1-2 hours at room temperature. Cap unreacted sites with DCM/methanol/DIPEA.
Q3: How should I cleave the product from the resin?
A3: Cleave with 0.5-1% TFA in DCM for 5-10 minutes, repeated 2-3 times. Neutralize the cleavage solution with pyridine or DIPEA.
Q4: How should I store 2-CTC Resin?
A4: Store powder at -20ˇăC for up to 3 years or at 4ˇăC for up to 2 years. Keep under anhydrous conditions to prevent hydrolysis.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of solid-phase synthesis, optimization of coupling and cleavage conditions is recommended for specific sequences.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-03151309
Product Name: 2-CTC Resin
Size: 10 g / 50 g / 100 g
Suggested Retail Price:
10 g: CNY ¥750.00 / USD $75.00 / EUR €90.00 / JPY ¥13500.00
50 g: CNY ¥1750.00 / USD $175.00 / EUR €210.00 / JPY ¥31500.00
100 g: CNY ¥2600.00 / USD $260.00 / EUR €312.00 / JPY ¥46800.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
