Calcitriol, 32222-06-3, IC-01242751
Product Introduction
Calcitriol (1¦Á,25-dihydroxyvitamin D3) is the biologically active form of vitamin D3 and the natural ligand for the vitamin D receptor (VDR). It is a secosteroid hormone that plays a central role in calcium and phosphate homeostasis by promoting intestinal calcium absorption, renal calcium reabsorption, and bone mineralization. Beyond its classical role in mineral metabolism, calcitriol regulates cell proliferation, differentiation, and immune function through VDR-mediated transcriptional regulation. It is widely used in research on osteoporosis, chronic kidney disease, secondary hyperparathyroidism, cancer, and immune regulation. Calcitriol also serves as a reference compound for studying vitamin D metabolism and VDR signaling pathways.
Product Features
1. Active Vitamin D3 Metabolite: The biologically active form of vitamin D3 and natural VDR ligand.
2. Calcium and Phosphate Homeostasis: Promotes intestinal calcium absorption and renal calcium reabsorption.
3. VDR-Mediated Transcriptional Regulation: Modulates cell proliferation, differentiation, and immune function.
4. Broad Research Utility: Applied in bone metabolism, kidney disease, cancer, and immunology research.
5. High Purity: Supplied at 98% purity for reproducible pharmacological studies.
Specifications
Size: 1 mg / 5 mg / 25 mg
CAS Number: 32222-06-3
Purity: 98%
Molecular Weight: 416.64
Molecular Formula: C27H44O3
Solubility: DMSO: ¡Ý50 mg/mL; Ethanol: ¡Ý20 mg/mL; limited solubility in aqueous buffers
Storage and Stability
Storage Conditions: Store at -20¡ãC under inert atmosphere, protected from light and moisture.
Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed. For reconstituted solutions, aliquot and store at -20¡ãC protected from light. Avoid repeated freeze-thaw cycles.
Protocol (For Reference Only)
Important: Calcitriol is light-sensitive and oxygen-sensitive. Prepare stock solutions in anhydrous DMSO under inert atmosphere. Protect from light during handling and storage.
1. Stock Solution Preparation: Dissolve calcitriol in anhydrous DMSO to prepare a concentrated stock solution (e.g., 10-50 mM). Aliquot into single-use vials and store at -20¡ãC protected from light under inert atmosphere.
2. In Vitro Working Solution Preparation: Dilute DMSO stock solution into culture medium to achieve working concentrations typically ranging from 1-100 nM. Limit final DMSO concentration to ¡Ü0.1%.
3. VDR Activation Assay: Treat VDR-expressing cells with calcitriol at 1-100 nM for 24-48 hours. Assess VDR target gene expression (e.g., CYP24A1, cathelicidin) by qPCR or Western blot.
4. Cell Proliferation and Differentiation Assays: Treat cells with calcitriol at 10-100 nM and assess effects on proliferation, differentiation, and apoptosis using standard assays.
5. In Vivo Studies: Calcitriol has been administered in rodent models; optimize dosing and route based on specific experimental requirements and published literature.
Precautions
1. Protect from light and moisture during handling and storage.
2. Calcitriol is a potent hormone; handle with appropriate safety precautions.
3. Use appropriate vehicle controls in all experiments; DMSO concentration in culture medium should not exceed 0.1%.
4. For in vivo studies, optimize dosing to avoid hypercalcemia.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the mechanism of action of calcitriol?
A1: Calcitriol binds to the vitamin D receptor (VDR) and regulates transcription of target genes involved in calcium and phosphate homeostasis, cell proliferation, differentiation, and immune function.
Q2: What is the recommended concentration for in vitro studies?
A2: Typical working concentrations range from 1-100 nM. Optimize for each cell type and endpoint.
Q3: How should I store calcitriol?
A3: Store at -20¡ãC under inert atmosphere, protected from light and moisture. Reconstituted stock solutions should be aliquoted and stored at -20¡ãC protected from light.
Q4: What precautions should I take for in vivo studies?
A4: Optimize dosing to avoid hypercalcemia, and monitor serum calcium levels in treated animals.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of conditions is recommended for specific experimental systems.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-01242751
Product Name: Calcitriol
Size: 1 mg / 5 mg / 25 mg
Suggested Retail Price:
1 mg: CNY ¥675.00 / USD $67.50 / EUR €81.00 / JPY ¥12150.00
5 mg: CNY ¥2025.00 / USD $202.50 / EUR €243.00 / JPY ¥36450.00
25 mg: CNY ¥8100.00 / USD $810.00 / EUR €972.00 / JPY ¥145800.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
