IPBlot HRP-conjugated Mouse Anti-Rabbit Native IgG, SA-10061

Product Introduction

This product is a mouse monoclonal antibody conjugated to high-activity horseradish peroxidase (HRP) that specifically recognizes rabbit native IgG. In co-immunoprecipitation (co-IP) assays, denatured IgG from IP samples may cause interference, resulting in immunoblotting signals at approximately 55 kDa (heavy chain) and approximately 25 kDa (light chain) when using traditional secondary antibodies. Products of the IPBlot series are designed for native IgG detection and are suitable for reducing interference from denatured IgG in IP/co-IP immunoblotting applications.

Product Features

1. Native IgG Specificity: Designed for detection of native rabbit IgG, reducing interference from denatured IgG heavy and light chains in IP/co-IP immunoblotting.

2. High-Activity HRP Conjugate: Conjugated with high-activity horseradish peroxidase for sensitive detection.

3. Affinity Purified: Purified by affinity chromatography for high specificity.

4. Broad Application: Suitable for Western blot and ELISA applications.

5. Ready-to-Use Liquid Format: Supplied in stabilized buffer with BSA and glycerol for convenient handling.

Specifications

Size: 50 µL / 250 µL

Host Species: Mouse

Target Species: Rabbit

Immunogen: Rabbit Native IgG

Clonality: Monoclonal Antibody

Conjugate: Horseradish Peroxidase (HRP)

Purification: Affinity Purified

Form: Liquid

Storage Buffer: Liquid in phosphate buffered saline containing 1% BSA, 0.01% thimerosal, 50% glycerol, pH 7.4

Recommended Dilution:

WB: 1:2,500 - 1:10,000

ELISA: 1:1,000 - 1:3,000

Research Field: Immunology

Storage and Stability

Storage Conditions: Protect from light. Store at 4¡ãC for short-term use. Store at -20¡ãC for long-term preservation. Avoid repeated freeze/thaw cycles.

Shelf Life: The product is stable for 12 months from the date of manufacture when stored as directed.

Protocol (For Reference Only)

Important: Protect HRP-conjugated antibody from prolonged light exposure. Centrifuge the vial briefly before opening. The optimal working dilution should be determined empirically for each application.

1. Western Blot (WB): Following membrane blocking and primary antibody incubation, wash the membrane thoroughly. Dilute IPBlot HRP-conjugated Mouse Anti-Rabbit Native IgG at 1:2,500 to 1:10,000 in blocking buffer. Incubate for 1 hour at room temperature with gentle agitation. Wash 3-5 times with TBST and detect using ECL substrate.

2. ELISA: Following coating, blocking, and primary antibody incubation, wash the plate thoroughly. Dilute the HRP-conjugated antibody at 1:1,000 to 1:3,000 in blocking buffer. Incubate for 1 hour at room temperature. Wash and detect using TMB or other appropriate HRP substrate.

3. IP/Co-IP Immunoblotting: Following immunoprecipitation and SDS-PAGE, transfer proteins to the membrane and use this antibody for detection of native IgG according to the Western blot procedure above.

Precautions

1. Protect from light to prevent HRP inactivation.

2. Avoid repeated freeze/thaw cycles; aliquot upon delivery and store at -20¡ãC.

3. The recommended dilution ranges are guidelines; optimal dilution must be determined empirically.

4. Contains thimerosal as a preservative; handle with appropriate precautions.

5. For research use only. Not for use in diagnostic or therapeutic procedures.

FAQ (Simplified)

Q1: What is the advantage of this antibody over traditional secondary antibodies in IP/Co-IP?

A1: Traditional secondary antibodies detect both native and denatured IgG, producing interfering bands at approximately 55 kDa and 25 kDa in IP/Co-IP immunoblotting. This IPBlot series antibody is designed for native IgG detection, reducing interference from denatured IgG.

Q2: What dilution should I use for Western blot?

A2: The recommended dilution range for Western blot is 1:2,500 to 1:10,000. Optimize based on your specific sample and detection system.

Q3: What dilution should I use for ELISA?

A3: The recommended dilution range for ELISA is 1:1,000 to 1:3,000. Optimize based on your specific assay.

Q4: How should I store this product?

A4: Store at 4¡ãC for short-term use or at -20¡ãC for long-term preservation, protected from light. Avoid repeated freeze/thaw cycles.

Disclaimer

1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.

2. Due to the variable nature of biological research, optimization of antibody dilution and experimental conditions is recommended.

3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.

4. Wear appropriate protective clothing and gloves when handling this product.

Ordering Information

Catalog Number: SA-10061

Product Name: IPBlot HRP-conjugated Mouse Anti-Rabbit Native IgG

Size: 50 µL / 250 µL

Suggested Retail Price:

50 µL: CNY ¥1200.00 / USD $120.00 / EUR €144.00 / JPY ¥21600.00

250 µL: CNY ¥3500.00 / USD $350.00 / EUR €420.00 / JPY ¥63000.00

Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.