Nile Red,7385-67-3, IC-03356831
Product Introduction
Nile Red is a fluorescent lipophilic dye widely used for the detection and quantification of intracellular neutral lipids and lipid droplets. It exhibits environment-dependent fluorescence: in non-polar lipid-rich environments, Nile Red emits strong yellow-gold fluorescence (Ex/Em ~552/636 nm), whereas in polar environments it emits weak red fluorescence. This property makes Nile Red a valuable tool for studying lipid metabolism, adipocyte differentiation, steatosis, and lipid droplet dynamics in cells and tissues. Nile Red is compatible with fluorescence microscopy, flow cytometry, and microplate reader-based assays, and is suitable for both live-cell and fixed-cell imaging applications.
Product Features
1. Lipophilic Fluorescence: Strong yellow-gold fluorescence in lipid-rich environments, enabling sensitive detection of neutral lipids.
2. Environment-Sensitive: Fluorescence emission varies with polarity, allowing discrimination of lipid droplets from other cellular structures.
3. Broad Application: Suitable for fluorescence microscopy, flow cytometry, and microplate-based assays.
4. Live-Cell Compatible: Can be used for live-cell imaging of lipid droplets.
5. High Purity: Supplied at 98% purity for reproducible staining.
Specifications
Size: 5 mg / 10 mg / 1 mL (10 mM in DMSO) / 25 mg / 50 mg
CAS Number: 7385-67-3
Purity: 98%
Molecular Weight: 318.37
Molecular Formula: C20H18N2O2
Excitation/Emission: ~552 nm / ~636 nm (lipid environment)
Solubility: DMSO: ¡Ý10 mg/mL; Ethanol: ¡Ý5 mg/mL; limited solubility in aqueous buffers
Recommended Working Concentration: 200-1000 nM
Storage and Stability
Storage Conditions: Store at 4¡ãC, protected from light. For solutions in solvent: store at -80¡ãC for 2 years or -20¡ãC for 1 year (protected from light).
Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed in powder form.
Protocol (For Reference Only)
Important: Protect from light throughout the procedure. Prepare working solution fresh before use.
1. Preparation of Working Solution: Dilute the stock solution with pre-warmed serum-free cell culture medium or PBS to a final concentration of 200-1000 nM. Note: Adjust the working concentration according to experimental requirements; prepare fresh before use.
2. Cell Staining:
2.1 Suspension cells: Collect cells by centrifugation, add PBS, and wash twice for 5 minutes each. Adherent cells: Discard culture medium, add trypsin to digest cells, centrifuge and discard supernatant, then add PBS and wash twice for 5 minutes each.
2.2 Add 1 mL of Nile Red working solution and incubate at room temperature for 5-10 minutes.
2.3 Centrifuge at 400 x g for 3-4 minutes at 4¡ãC and discard supernatant.
2.4 Add PBS to wash cells twice for 5 minutes each.
2.5 Resuspend cells in 1 mL of serum-free medium or PBS and observe using a fluorescence microscope.
Precautions
1. Protect from light throughout the procedure.
2. Prepare working solution fresh before use.
3. Adjust working concentration according to cell type and experimental requirements.
4. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the recommended working concentration of Nile Red?
A1: The recommended working concentration is 200-1000 nM. Optimize according to cell type and experimental requirements.
Q2: What is the excitation/emission of Nile Red?
A2: In lipid-rich environments, Nile Red has excitation/emission maxima of approximately 552 nm/636 nm. Fluorescence emission varies with environment polarity.
Q3: How should I store Nile Red?
A3: Store powder at 4¡ãC protected from light. For solutions, store at -80¡ãC for 2 years or -20¡ãC for 1 year protected from light.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of staining conditions is recommended for specific cell types.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-03356831
Product Name: Nile Red
Size: 5 mg / 10 mg / 1 mL (10 mM in DMSO) / 25 mg / 50 mg
Suggested Retail Price:
5 mg: CNY ¥385.00 / USD $38.50 / EUR €46.20 / JPY ¥6030.00
10 mg: CNY ¥540.00 / USD $54.00 / EUR €64.80 / JPY ¥9720.00
1 mL (10 mM in DMSO): CNY ¥595.00 / USD $59.50 / EUR €71.40 / JPY ¥10710.00
25 mg: CNY ¥675.00 / USD $67.50 / EUR €81.00 / JPY ¥12150.00
50 mg: CNY ¥875.00 / USD $87.50 / EUR €105.00 / JPY ¥15750.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
