L-Lactate Dehydrogenase, E. coli, IC-03997865
Product Introduction
L-Lactate dehydrogenase (L-LDH) from Escherichia coli is a redox enzyme that catalyzes the reduction of pyruvate to L-lactate by NADH in vivo with absolute enantiospecificity. It is widely used in biochemical research, chiral synthesis, and enzymatic assays for lactate detection and quantification.
Product Features
1. High Enantiospecificity: Catalyzes the reduction of pyruvate to L-lactate with absolute enantiospecificity.
2. High Specific Activity: ≥30 U/mg solid.
3. Good Solubility: Solubility in H₂O ≥100 mg/mL.
4. Well-Characterized Properties: Defined molecular weight, isoelectric point, optimal pH and temperature, and stability profiles.
5. Ready-to-Use: Soluble in pure water for convenient preparation.
Specifications
Size: 5 mg / 10 mg / 50 mg
CAS Number: 9001-60-9
Specific Activity: ≥30 U/mg solid
Solubility: ≥100 mg/mL in H₂O
Molecular Weight: 38 kDa (SDS-PAGE)
Isoelectric Point: 6.2
Optimal pH: 6.5
Optimal Temperature: 45°C
pH Stability: 4.5-10.0 (37°C, 1 hour)
Thermal Stability: <50°C (pH 7.4, 15 minutes)
Inhibitors: Co²⁺, Cu²⁺, Fe³⁺, Ni²⁺, Zn²⁺, NEM, SDS, Proclin
Storage and Stability
Storage Conditions: Store at -20°C for 2 years.
Shelf Life: 2 years from the date of manufacture when stored at -20°C as directed.
Protocol (For Reference Only)
Important: Dissolve in pure water. Avoid repeated freeze-thaw cycles.
1. Reconstitution: Dissolve L-Lactate dehydrogenase in pure water to the desired concentration. Mix gently; do not vortex vigorously.
2. Enzymatic Assay: Prepare reaction buffer at the optimal pH (6.5) containing NADH and pyruvate. Initiate the reaction by adding L-LDH. Monitor NADH oxidation spectrophotometrically at 340 nm.
3. Reaction Conditions: Optimal temperature is 45°C and optimal pH is 6.5. For standard assays, incubate at 37°C or as appropriate for the specific application.
4. Storage of Working Solution: Aliquot the reconstituted enzyme into single-use volumes and store at -20°C. Avoid repeated freeze-thaw cycles.
Precautions
1. Avoid contact with inhibitors including Co²⁺, Cu²⁺, Fe³⁺, Ni²⁺, Zn²⁺, NEM, SDS, and Proclin.
2. Dissolve in pure water; avoid buffers containing inhibitory metal ions.
3. Aliquot into single-use volumes to avoid repeated freeze-thaw cycles.
4. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the optimal pH and temperature for this enzyme?
A1: The optimal pH is 6.5 and the optimal temperature is 45°C. For standard assays, 37°C may be used.
Q2: What are the inhibitors of this enzyme?
A2: Inhibitors include Co²⁺, Cu²⁺, Fe³⁺, Ni²⁺, Zn²⁺, NEM, SDS, and Proclin.
Q3: How should I store this enzyme?
A3: Store at -20°C for up to 2 years. Aliquot reconstituted enzyme into single-use volumes to avoid repeated freeze-thaw cycles.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of assay conditions is recommended for specific applications.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-03997865
Product Name: L-Lactate Dehydrogenase, E. coli
Size: 5 mg / 10 mg / 50 mg
Suggested Retail Price:
5 mg: CNY ¥850.00 / USD $85.00 / EUR �102.00 / JPY ¥15300.00
10 mg: CNY ¥1150.00 / USD $115.00 / EUR �138.00 / JPY ¥20700.00
50 mg: CNY ¥4250.00 / USD $425.00 / EUR �510.00 / JPY ¥76500.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
