Thiazolyl Blue (MTT), IC-02108506
Product Introduction
Thiazolyl Blue Tetrazolium Bromide (MTT) is a yellow tetrazolium salt widely used for the colorimetric assessment of cell viability, proliferation, and cytotoxicity. The assay principle is based on the ability of metabolically active cells to reduce MTT to insoluble purple formazan crystals through the action of mitochondrial succinate dehydrogenase and other oxidoreductases. The amount of formazan produced is directly proportional to the number of viable cells, as dead cells lack the metabolic activity required for this reduction. The formazan crystals are solubilized in DMSO, isopropanol, or detergent-containing solutions, and the resulting absorbance is measured spectrophotometrically at 570 nm. MTT is a standard reagent for cell proliferation assays, drug sensitivity testing, and cytotoxicity evaluation in cancer research, immunology, and drug discovery.
Product Features
1. Standard Viability Assay Reagent: Widely used for cell proliferation, viability, and cytotoxicity assessment.
2. Metabolic Activity-Based: Measures mitochondrial dehydrogenase activity as an indicator of viable cell number.
3. Simple and Reproducible: Simple protocol with colorimetric readout compatible with standard microplate readers.
4. Broad Application: Suitable for adherent and suspension cells, high-throughput screening, and drug sensitivity testing.
5. High Purity: Supplied at ≥98% purity for reproducible assay performance.
Specifications
Size: 250 mg
CAS Number: 298-93-1
Molecular Weight: 414.32
Molecular Formula: C18H16BrN5S
Purity: ≥98%
Appearance: Yellow to orange-yellow powder
Solubility: Water: ~10 mg/mL; Ethanol: slightly soluble; DMSO: soluble
Storage and Stability
Storage Conditions: Store at -20°C, protected from light and moisture.
Shelf Life: The product is stable for 36 months from the date of manufacture when stored as directed. Protect from light; MTT is light-sensitive.
Protocol (For Reference Only)
Important: MTT is light-sensitive. Protect solutions and plates from light. MTT is a potential irritant; handle with appropriate precautions.
1. Stock Solution Preparation: Dissolve MTT in sterile PBS or culture medium without serum to a concentration of 5 mg/mL. Filter sterilize (0.22 µm) and protect from light. Store at 4°C for up to 2 weeks or aliquot and store at -20°C for longer periods.
2. Cell Seeding and Treatment: Seed cells in 96-well plates at an appropriate density (typically 1×10³ - 1×10⁴ cells per well) and allow to attach overnight. Treat cells with test compounds as required.
3. MTT Addition: Add 10-20 µL of MTT stock solution (5 mg/mL) per 100 µL culture medium. Incubate at 37°C for 2-4 hours protected from light.
4. Formazan Solubilization: Carefully aspirate the medium without disturbing the formazan crystals. Add 100-150 µL of DMSO or isopropanol per well. Shake gently for 10-15 minutes to dissolve the crystals completely.
5. Absorbance Measurement: Measure absorbance at 570 nm using a microplate reader. Subtract background absorbance if required. Cell viability is proportional to the absorbance value.
Precautions
1. Protect MTT solutions and plates from light throughout the procedure.
2. MTT is a potential irritant and should be handled with gloves in a well-ventilated area.
3. Formazan crystals should be completely dissolved before absorbance measurement.
4. For suspension cells, centrifuge the plate before aspirating medium to prevent cell loss.
5. For research use only. Not for use in diagnostic or therapeutic procedures.
FAQ (Simplified)
Q1: What is the detection principle of the MTT assay?
A1: Metabolically active cells reduce MTT to purple formazan crystals via mitochondrial dehydrogenases. The amount of formazan produced is proportional to the number of viable cells.
Q2: What is the recommended MTT concentration?
A2: A final concentration of 0.5 mg/mL in culture medium is commonly used. Add 10 µL of 5 mg/mL stock per 100 µL medium.
Q3: How should I store MTT?
A3: Store powder at -20°C protected from light and moisture. Prepare stock solutions in sterile PBS at 5 mg/mL, filter sterilize, and store at 4°C for up to 2 weeks or at -20°C for longer periods.
Disclaimer
1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.
2. Due to the variable nature of biological research, optimization of cell density and incubation time is recommended for specific cell types.
3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.
4. Wear appropriate protective clothing and gloves when handling this product.
Ordering Information
Catalog Number: IC-02108506
Product Name: Thiazolyl Blue (MTT)
Size: 250 mg
Suggested Retail Price: CNY ¥550.00 / USD $55.00 / EUR �66.00 / JPY ¥9900.00
Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.
