Ponceau S Staining Solution, Ready-to-Use, IC-8004

Product Introduction

This product is a ready-to-use Ponceau S staining solution designed for the rapid and reversible detection of proteins on nitrocellulose (NC) and PVDF membranes after Western blot transfer. Ponceau S is a red anionic diazo dye that binds reversibly to positively charged amino acid residues and the peptide backbone of proteins, producing visible red bands on the membrane. This staining allows for quick visual assessment of transfer efficiency, evenness of protein loading, and overall protein integrity before proceeding to immunodetection. The dye can be easily removed by washing with water or TBST, making it fully compatible with subsequent blocking and antibody incubation steps. This ready-to-use formulation eliminates the need for dye powder weighing and dissolution, providing a convenient and reproducible solution for routine Western blot quality control.

Product Features

1. Ready-to-Use Convenience: No preparation required, open and use directly.

2. Rapid Staining: Protein bands visible within 1-5 minutes at room temperature.

3. Reversible Staining: Dye is easily removed by washing with water or TBST, fully compatible with subsequent immunodetection.

4. Broad Membrane Compatibility: Suitable for nitrocellulose (NC) and PVDF membranes.

5. Sensitive Detection: Detects protein bands with sensitivity comparable to standard Ponceau S formulations.

Specifications

Size: 100 mL

Application: Reversible protein staining on NC and PVDF membranes after Western blot transfer

Formulation: Ready-to-use solution

Detection Method: Visual (red bands on membrane)

Storage and Stability

Storage Conditions: Store at room temperature (15-25¡ãC), protected from light.

Shelf Life: The product is stable for 12 months from the date of manufacture when stored as directed. After opening, use within 12 months when stored properly.

Protocol (For Reference Only)

Important: Handle membranes with clean forceps. Ensure the membrane remains moist throughout the staining and destaining procedure. Perform staining in a clean container.

1. Post-Transfer Staining: After protein transfer, rinse the membrane briefly in deionized water to remove residual transfer buffer.

2. Staining: Immerse the membrane in sufficient Ponceau S Staining Solution to completely cover the membrane surface. Incubate at room temperature for 1-5 minutes with gentle agitation until protein bands become clearly visible.

3. Destaining and Documentation: Pour off the staining solution (may be reused). Rinse the membrane with deionized water several times to remove excess dye and enhance band contrast. Photograph or scan the membrane for documentation.

4. Complete Destaining: Wash the membrane with TBST or PBS for 5-10 minutes with agitation to completely remove the dye. Alternatively, continue with blocking directly as residual dye will be removed during subsequent washing steps. Proceed with standard blocking and immunodetection protocols.

Precautions

1. The staining solution may be reused several times. Discard when staining intensity decreases or solution becomes contaminated.

2. Ponceau S staining is less sensitive than permanent staining methods such as Coomassie Blue; faint bands may not be visible.

3. For PVDF membranes, ensure the membrane is pre-wetted with methanol before staining.

4. For research use only. Not for use in diagnostic or therapeutic procedures.

FAQ (Simplified)

Q1: Does Ponceau S interfere with subsequent antibody detection?

A1: No. Ponceau S is completely reversible and is easily removed by washing with water or TBST. It does not interfere with blocking or antibody binding.

Q2: How sensitive is Ponceau S staining?

A2: Ponceau S typically detects protein bands down to 50-100 ng per band, depending on the protein. It is less sensitive than Coomassie Blue or silver staining but sufficient for routine transfer quality assessment.

Q3: Can I reuse the staining solution?

A3: Yes. The solution may be reused several times. Store at room temperature and discard when staining intensity decreases.

Q4: How should I document the staining results?

A4: Rinse the membrane briefly in water to enhance band contrast, then photograph or scan the membrane immediately. The dye will fade during subsequent washing steps.

Disclaimer

1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.

2. Due to the variable nature of biological samples, staining intensity may vary depending on protein amount and composition.

3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.

4. Wear appropriate protective clothing and gloves when handling this product.

Ordering Information

Catalog Number: IC-8004

Product Name: Ponceau S Staining Solution, Ready-to-Use

Size: 100 mL

Suggested Retail Price: CNY ¥220.00 / USD $22.00 / EUR €26.40 / JPY ¥3960.00

Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.