Western Blot Wash Buffer, 10X (TBST, 10X), IC-8020

Product Introduction

This product is a 10X concentrated stock solution of TBST (Tris-Buffered Saline with Tween 20), formulated as a ready-to-dilute wash buffer for Western blot membrane washing steps. TBST is the standard wash buffer used in Western blotting, ELISA, and other immunodetection techniques, serving to remove unbound primary and secondary antibodies and reduce non-specific binding. The inclusion of Tween 20 as a non-ionic detergent minimizes non-specific protein-protein interactions between antibodies and the membrane surface, reducing background signal and improving detection specificity. This 10X concentrated formulation provides convenience for long-term storage and allows rapid preparation of 1X working solutions by simple dilution with deionized water, ensuring consistent pH and ionic strength across experiments.

Product Features

1. 10X Concentrated Format: Convenient storage and cost-effective, diluted to 1X before use.

2. Standard TBST Formulation: Optimized Tris-buffered saline with Tween 20 for reliable wash performance.

3. Ready-to-Dilute: Simple 1:10 dilution with deionized water to prepare 1X working solution.

4. Versatile Application: Suitable for Western blot, ELISA, and other immunodetection washing steps.

Specifications

Size: 500 mL

Concentration: 10X

Application: Western blot membrane washing, ELISA plate washing

Formulation: 10X TBST (Tris-Buffered Saline with Tween 20)

Storage and Stability

Storage Conditions: Store at room temperature (15-25¡æ). If precipitation occurs at low temperatures, warm to room temperature and mix before use.

Shelf Life: The product is stable for 24 months from the date of manufacture when stored as directed.

Protocol (For Reference Only)

Important: Ensure any precipitate is fully dissolved before dilution. Prepare 1X working solution fresh for optimal results.

1. Preparation of 1X Working Solution: Dilute the 10X TBST 1:10 with deionized water (e.g., 100 mL of 10X TBST plus 900 mL of deionized water). Mix thoroughly by stirring or inversion.

2. Western Blot Washing: After primary or secondary antibody incubation, wash the membrane with 1X TBST 3-5 times, 5-10 minutes each, with vigorous agitation.

3. ELISA Washing: After each antibody incubation step, wash the plate with 1X TBST 3-5 times.

4. Storage of 1X Solution: 1X TBST may be stored at room temperature and used within 1-2 weeks.

Precautions

1. If precipitation occurs during cold storage, warm the bottle to room temperature and mix until the precipitate fully dissolves before dilution.

2. Do not use the 10X concentrate directly; always dilute to 1X before use.

3. 1X working solution is recommended to be prepared fresh. If stored, use within 1-2 weeks.

4. For research use only. Not for use in diagnostic or therapeutic procedures.

FAQ (Simplified)

Q1: What should I do if precipitate forms in the 10X concentrate?

A1: Precipitation may occur at low temperatures. Warm the bottle to room temperature and mix until the precipitate completely dissolves before use.

Q2: Can I reuse 1X TBST?

A2: 1X TBST is typically used once for optimal wash performance. Reuse is not recommended as detergent concentration may decrease and contaminants may accumulate.

Q3: What is the recommended wash protocol?

A3: For Western blot, wash 3-5 times, 5-10 minutes each, with vigorous agitation after each antibody incubation step.

Disclaimer

1. For Research Use Only. Not for use in diagnostic or therapeutic procedures.

2. Due to the variable nature of biological research, optimization of wash conditions may be required for specific antibodies and applications.

3. This warranty is limited to the replacement of the product. The manufacturer assumes no liability for incidental or consequential damages.

4. Wear appropriate protective clothing and gloves when handling this product.

Ordering Information

Catalog Number: IC-8020

Product Name: Western Blot Wash Buffer, 10X (TBST, 10X)

Size: 500 mL

Suggested Retail Price: CNY ¥200.00 / USD $20.00 / EUR €24.00 / JPY ¥3600.00

Note: The specific price is subject to the transaction price in the offline contract. This document is provided for technical reference only and does not constitute any form of sales advertisement or invitation to offer. For product details and purchase inquiries, please contact local sales representatives.